FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48481
Sequences flagged as poor quality0
Sequence length301
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCTA950.0295.000032
TGACCTA950.0295.000032
TCGCCTA1000.0295.02
TTCTACG108.4400654E-4295.03
GTCGCCT350.0295.01
ACGCCTA350.0295.02
ACGCCCT157.1977247E-6295.01
TCCGCCT206.1243554E-8295.01
GCGCGAA255.2386895E-10295.0295
ACCGCCT303.6379788E-12295.01
GTCCTAC2300.0295.03
GATCCTA450.0295.02
GGTCCTA750.0295.02
ATAGCCT206.1243554E-8295.01
CGACCCT157.1977247E-6295.01
GCGCCTA350.0295.02
TCCTACG12350.0295.04
TCCTAAG108.4400654E-4295.04
GCACCTA800.0295.02
CCGTCCT108.4400654E-4295.01
Produced by FastQC (version 0.11.5)