FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98916
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACCT550.0295.000031
TGATCCT500.0295.01
TCGCCTA1450.0295.02
GTCGCCT450.0295.01
CCCCTAG108.453355E-4295.03
ACGCCTA1350.0295.02
ACGCCCT350.0295.01
GTTTCCT600.0295.01
TTGTCCT305.456968E-12295.01
CGCGCCT206.1565515E-8295.01
CCTACGC108.453355E-4295.05
GCGCCTA1000.0295.02
TGACCTA1200.0295.02
TACGCCT450.0295.01
GTTCCCT255.275069E-10295.01
CTCGCCT450.0295.01
CCGACCT255.275069E-10295.01
TTGCCCT500.0295.01
GTCCCCT450.0295.01
GACGCCT500.0295.01
Produced by FastQC (version 0.11.5)