FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71977
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA900.0295.02
GCCCCTA700.0295.02
GTCGCCT255.2568794E-10295.01
TGCCCCT255.2568794E-10295.01
CGAACCT303.6379788E-12295.01
TCACCCT303.6379788E-12295.01
CTATCCT255.2568794E-10295.01
CGACCTA1000.0295.02
GGATCCT350.0295.01
CGACCCT303.6379788E-12295.01
CCTACGA255.2568794E-10295.05
ACGGGCG157.2122457E-6295.07
GCACCCT350.0295.01
TTCCCTA1000.0295.02
AAGCCTA900.0295.02
TGACCTA1350.0295.02
ACCTAGG157.2122457E-6295.04
TACGCCT303.6379788E-12295.01
CTACGAG255.2568794E-10295.06
ATCCCCT303.6379788E-12295.01
Produced by FastQC (version 0.11.5)