FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106598
Sequences flagged as poor quality0
Sequence length301
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACCT450.0295.01
GACGCCT305.456968E-12295.01
CGATCCT305.456968E-12295.01
GTGCCTA950.0295.02
CGCCTCG108.454272E-4294.999973
TTCGCCT206.158734E-8294.999971
GGCTACG108.454272E-4294.999973
CCTACGG99200.0290.093235
GCCTACG18950.0289.551454
TCCTACG29350.0289.47194
CCCTACG23800.0288.802524
ACCTACG27900.0288.65594
CTACGGG96950.0288.305856
ACGGGAG95400.0288.197088
TACGGGA95700.0288.064247
ACGCCTA1550.0285.483862
CGACCTA1500.0285.16672
GCCCCTA1450.0284.827582
CGGGAGG94650.0284.247229
GTCCTAC6750.0284.074073
Produced by FastQC (version 0.11.5)