FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68997
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCTA850.0295.000032
ACGCCTA850.0295.000032
GGTCCTA1100.0295.000032
GCGCCTA850.0295.000032
ACAGCCT550.0295.000031
GAGCCTA550.0295.000032
GTGCCTA550.0295.000032
TGATCCT350.0295.01
TCGCCCT157.2109415E-6295.01
ATCCTAG108.4478105E-4295.03
AGATCCT450.0295.01
CGAACCT157.2109415E-6295.01
GCGACCT206.143091E-8295.01
TCACCCT450.0295.01
GTTTCCT350.0295.01
AGCCCTA1050.0295.02
TAGACCT206.143091E-8295.01
ACCGCCT108.4478105E-4295.01
GTCCTAC4500.0295.03
CTCCCTA900.0295.02
Produced by FastQC (version 0.11.5)