FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46595
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC660.14164609936688485No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC630.1352076403047537No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC600.1287691812426226No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC580.12447687520120185No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC570.12233072218049149No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC570.12233072218049149No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC570.12233072218049149No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTC540.11589226311836034No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC530.11374611009764997No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC530.11374611009764997No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC520.11159995707693958No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC520.11159995707693958No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC520.11159995707693958No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC510.10945380405622922No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC510.10945380405622922No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC500.10730765103551883No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC500.10730765103551883No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTC500.10730765103551883No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC500.10730765103551883No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC500.10730765103551883No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC500.10730765103551883No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC490.10516149801480847No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC490.10516149801480847No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTC480.10301534499409808No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTC480.10301534499409808No Hit
TCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC480.10301534499409808No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC480.10301534499409808No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC480.10301534499409808No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC480.10301534499409808No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC480.10301534499409808No Hit
CATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC470.10086919197338771No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC470.10086919197338771No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTC470.10086919197338771No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC470.10086919197338771No Hit
TGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC470.10086919197338771No Hit
TTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC470.10086919197338771No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCGG350.0295.00003295
TTACCTA700.0295.000032
TAGCCTA850.0295.000032
GTCCCTA700.0295.000032
CGAACCT350.0295.000031
CTGCCTA350.0295.000032
AGTCCTA850.0295.000032
CGTCCTA350.0295.000032
GTAGCCT350.0295.000031
AACACCT350.0295.000031
CAGCCTA550.0295.000032
CGTTCCT108.439002E-4295.01
ATCCCCT108.439002E-4295.01
AGCCTAC2450.0295.03
GCGGGAG108.439002E-4295.08
GTTGATT500.0295.0295
GTTTGTC108.439002E-4295.0295
TTGTCCT206.121809E-8295.01
AACGCCT206.121809E-8295.01
ACGCCTA303.6379788E-12295.02
Produced by FastQC (version 0.11.5)