FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103025
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCGG500.0294.99664295
ACGACCT255.275069E-10294.996641
TCGCCTA1600.0294.99662
TGCCCCT400.0294.99661
CGAACCT400.0294.99661
CGTGCCT157.2216117E-6294.99661
CACGCCT206.1581886E-8294.99661
CGCGCCT350.0294.99661
CCTACGT157.2216117E-6294.99665
GCGCCCT108.4541546E-4294.99661
CGTTCCT350.0294.99661
GTAGCCT305.456968E-12294.99661
CTCGGAG108.4541546E-4294.99666
CGCTACG108.4541546E-4294.99663
TCGTCCT450.0294.99661
CCGACCT350.0294.99661
TTGCCTA900.0294.99662
GACGCCT157.2216117E-6294.99661
GATTCCT157.2216117E-6294.99661
CGATCCT157.2216117E-6294.99661
Produced by FastQC (version 0.11.5)