FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82830
Sequences flagged as poor quality0
Sequence length301
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA1300.0295.02
GATCCTA1300.0295.02
GGCCCTA1150.0295.02
CGCTCCT350.0295.01
CTCGCCT108.450872E-4295.01
GACGCCT303.6379788E-12295.01
TTACCCT550.0295.01
TAGTCCT206.150549E-8295.01
GTGCCTA800.0295.02
GCCTACG15350.0293.07824
CCTACGG78000.0291.028845
CTACGGG75450.0290.699166
CCCTACG19600.0290.484684
TACGGGA74350.0290.238747
TCCTACG22500.0289.755554
ACGGGAG74800.0288.492658
ACCTACG21250.0286.67064
GTCCTAC5050.0286.237643
CGGGAGG73650.0285.78759
CGTCCTA1550.0285.483862
Produced by FastQC (version 0.11.5)