FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67920
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.10011778563015312No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.10011778563015312No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCT350.0295.000031
GCGCCTA700.0295.000032
CGATCCT350.0295.000031
GTGCCTA700.0295.000032
TCGCCTA900.0295.02
GCCCCTA800.0295.02
GTCGCCT303.6379788E-12295.01
CGTGCCT157.2104576E-6295.01
GGTCCTA850.0295.02
TTGTCCT400.0295.01
CACGCCT157.2104576E-6295.01
CGCGCCT157.2104576E-6295.01
CCTACGA108.4475265E-4295.05
GTCTACG108.4475265E-4295.04
ACGGGGC157.2104576E-6295.08
GACCCCT255.2568794E-10295.01
ATTCCCT255.2568794E-10295.01
AAGCCTA600.0295.02
TACGCCT255.2568794E-10295.01
TGCGCCT303.6379788E-12295.01
Produced by FastQC (version 0.11.5)