FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005491266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72421
Sequences flagged as poor quality0
Sequence length301
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA35364.882561687908203No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA24133.3319064912111127No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA17512.4178069896853125No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA17332.392952320459535No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA16892.3321964623520803No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA15952.2023998563952443No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA12991.7936786291269107No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA12591.738446030847406No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA10811.4926609685036107No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA10221.4111928860413414No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA9471.3076317642672706No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA8981.2399718313748773No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA8851.2220212369340384No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA8331.1502188591706826No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA8271.141933969428757No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA7711.0646083318374504No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA7501.0356112177407106No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA7491.0342304027837228No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATCCCCGCACTTTCGTGCCTCA6430.8878640173430359No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA6010.829869789149556No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA5930.8188232694936551No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5840.8063959348807666No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA5740.7925877853108905No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA5720.7898261553969153No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA5220.7207854075475345No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA5100.7042156280636832No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA5010.6917882934507947No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4730.6531254746551415No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4570.6310324353433396No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA4320.5965120614186493No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4320.5965120614186493No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4310.5951312464616617No Hit
GGACTACACGGGTATCTAATCCTGTTCGATCCCCGCACTTTCGTGCCTCA4300.5937504315046741No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA4130.5702765772358847No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA3880.5357562033111942No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA3870.5343753883542066No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA3840.5302329434832438No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA3770.5205672387843305No Hit
GGACTACAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG3560.4915701246875906No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA3510.4846660499026526No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3440.4750003452037393No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATCCCCGCACTTTCGTGCCTCA3380.4667154554618136No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA3300.45566893580591267No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATCCCCGCACTTTCGTGCCTCA3260.4501456759779622No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA3210.44324160119302414No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3130.4321950815371232No Hit
GGACTAAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG2980.411482857182309No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA2960.40872122726833376No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATCCCCGCACTTTCGTGCCTCA2940.4059595973543585No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA2940.4059595973543585No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATCCCCGCACTTTCGTGCCTCA2920.4031979674403833No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2810.3880090029135196No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA2780.3838665580425567No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2710.3742008533436434No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2640.36453514864473013No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2600.35901188881677965No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA2600.35901188881677965No Hit
GGACTACCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG2590.35763107385979204No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2560.3534886289888292No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2490.3438229242899159No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2460.3396804794189531No Hit
GGACTACAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG2450.33829966446196547No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2380.32863395976305215No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2370.32725314480606454No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA2330.32172988497811406No Hit
GGACTAAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG2330.32172988497811406No Hit
GGACTACGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG2250.31068336532221313No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATCCCCGCACTTTCGTGCCTCA2200.3037792905372751No Hit
GGACTAACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG2200.3037792905372751No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2160.29825603070932466No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2110.29135195592438656No Hit
GGACTACAAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCA2070.28582869609643613No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATCCCCGCACTTTCGTGCCTCA2070.28582869609643613No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2020.27892462131149803No Hit
GGACTAAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG1880.25959321191367146No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1860.2568315819996962No Hit
GGACTACACGGGTTTCTAATCCTGTTCGATCCCCGCACTTTCGTGCCTCA1860.2568315819996962No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1860.2568315819996962No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATCCCCGCACTTTCGTGCCTCA1840.254069952085721No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1820.25130832217174576No Hit
GGACTATAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG1820.25130832217174576No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1770.24440424738680766No Hit
GGACTACCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG1750.2416426174728325No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1710.236119357644882No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1550.21402631833308017No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1540.21264550337609256No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1520.20988387346211734No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATCCCCGCACTTTCGTGCCTCA1510.20850305850512973No Hit
GGACTACACGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCA1510.20850305850512973No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGATCCCCGCACTTTCGTGCCTCA1500.2071222435481421No Hit
GGACTACGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG1500.2071222435481421No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1490.2057414285911545No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1480.20436061363416688No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1460.20159898372019164No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1460.20159898372019164No Hit
GGACTAACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG1450.20021816876320403No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGATCCCCGCACTTTCGTGCCTCA1420.19607572389224118No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATCCCCGCACTTTCGTGCCTCA1420.19607572389224118No Hit
GGACTATCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG1400.193314093978266No Hit
GGACTATGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG1390.19193327902127835No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1320.18226757432236507No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1320.18226757432236507No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1320.18226757432236507No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1300.17950594440838982No Hit
GGACTAAGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG1230.16984023970947654No Hit
GGACTACCAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCA1210.16707860979550132No Hit
GGACTACAGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCA1180.16293616492453847No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATCCCCGCACTTTCGTGCCTCA1170.16155534996755086No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1170.16155534996755086No Hit
GGACTATAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG1150.15879372005357562No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1150.15879372005357562No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1130.1560320901396004No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1120.15465127518261276No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1110.15327046022562515No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1090.15050883031164994No Hit
GGACTACTAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCA1060.1463663854406871No Hit
GGACTAAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG1060.1463663854406871No Hit
GGACTACAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG1030.14222394056972426No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1020.14084312561273665No Hit
GGACTACAGGGTCTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG1010.13946231065574902No Hit
GGACTACAAGGGTTTCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCA1000.1380814956987614No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA990.1367006807417738No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA980.1353198657847862No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA970.13393905082779856No Hit
GGACTACAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG970.13393905082779856No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGATCCCCGCACTTTCGTGCCTCA960.13255823587081095No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA960.13255823587081095No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTCTCGTGCATCA930.1284157909998481No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATCCCCGCACTTTCGTGCCTCA910.12565416108587288No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA900.12427334612888526No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA900.12427334612888526No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA890.12289253117189765No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA880.12151171621491005No Hit
GGACTAAAGGGTCTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG860.11875008630093482No Hit
GGACTAAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG840.11598845638695959No Hit
GGACTACCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG840.11598845638695959No Hit
GGACTACTCGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCA840.11598845638695959No Hit
GGACTACGGGGTCTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG830.11460764142997197No Hit
GGACTATCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG800.11046519655900913No Hit
GGACTACCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG780.1077035666450339No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA780.1077035666450339No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA760.10494193673105867No Hit
GGACTACCGGGTCTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG750.10356112177407105No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA750.10356112177407105No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA730.10079949186009582No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA730.10079949186009582No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA730.10079949186009582No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)