FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77407
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC890.1149766816954539No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATT108.449808E-4295.0295
TCGCCCT206.148002E-8295.01
GTCGCCT255.2568794E-10295.01
CGAACCT350.0295.01
ACGCCTA850.0295.02
CGGCCTA800.0295.02
GATCCTA1150.0295.02
TTGTCCT650.0295.01
CACGCCT157.2143557E-6295.01
GGATCCT206.148002E-8295.01
CCTACGA206.148002E-8295.05
TCCTCGG108.449808E-4295.04
TAGCCTA1350.0295.02
ACGGGGC108.449808E-4295.08
ACCTCGG255.2568794E-10295.04
GTTACCT157.2143557E-6295.01
TTCCCTA1300.0295.02
CGGGGCA108.449808E-4295.09
CGCTCCT108.449808E-4295.01
CTCGGGA600.0295.06
Produced by FastQC (version 0.11.5)