FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58069
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA1100.0295.000032
GTCGCCT350.0295.000031
CCGGCCT350.0295.000031
GGGCCTA550.0295.000032
CTACCCT350.0295.000031
GGACCTA700.0295.000032
GGCCTAC2200.0295.000033
ATCGCCT350.0295.000031
TAGTCCT550.0295.000031
TGATCCT206.134724E-8295.01
AGTCCTA950.0295.02
CCCCTAC3450.0295.03
TGCCCCT255.2568794E-10295.01
ACGCCTA400.0295.02
ACGCCCT108.444373E-4295.01
GATCCTA1050.0295.02
ATAGCCT157.2050734E-6295.01
GCGCCCT157.2050734E-6295.01
TAGCCTA750.0295.02
CTGACCT157.2050734E-6295.01
Produced by FastQC (version 0.11.5)