FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68073
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.14102507602133002No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.13955606481277452No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC910.13368001997855244No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC880.12927298635288587No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC880.12927298635288587No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC870.12780397514433037No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC860.12633496393577484No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC850.12486595272721931No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC830.12192793031010826No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC810.11898990789299722No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC810.11898990789299722No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC800.11752089668444171No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC800.11752089668444171No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC800.11752089668444171No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC800.11752089668444171No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC780.11458287426733066No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC770.11311386305877513No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC770.11311386305877513No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC770.11311386305877513No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC760.11164485185021962No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC750.1101758406416641No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC750.1101758406416641No Hit
AATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC750.1101758406416641No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC740.10870682943310858No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.10870682943310858No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC730.10723781822455304No Hit
CCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC730.10723781822455304No Hit
AGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC720.10576880701599753No Hit
AGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC720.10576880701599753No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC720.10576880701599753No Hit
TGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC720.10576880701599753No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC710.104299795807442No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC700.10283078459888649No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC700.10283078459888649No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC700.10283078459888649No Hit
TAAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC700.10283078459888649No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC700.10283078459888649No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC690.10136177339033098No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC690.10136177339033098No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC690.10136177339033098No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACCTA1250.0295.000032
ATACCTA1250.0295.000032
TGATCCT500.0295.01
GCCCCTA750.0295.02
AATCCTA1000.0295.02
AATCCCT108.447568E-4295.01
ATCTCCT450.0295.01
CGAACCT206.142545E-8295.01
ACGCCTA800.0295.02
GTACAGT400.0295.0295
CACGGGA500.0295.06
CCTAGGA206.142545E-8295.05
AGCCCTA400.0295.02
GAGTCCT400.0295.01
AGCCCCT255.2568794E-10295.01
GTCCTAC4700.0295.03
ATTACCT206.142545E-8295.01
CTCCCTA800.0295.02
GGTCCTA1100.0295.02
TTGTCCT550.0295.01
Produced by FastQC (version 0.11.5)