FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48781
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.18654804124556693No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC840.17219819191898483No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC750.15374838564195076No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.15169840716672475No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC700.1434984932658207No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC680.1393985363153687No Hit
GGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC650.13324860088969065No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC650.13324860088969065No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC650.13324860088969065No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC640.13119862241446464No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC640.13119862241446464No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC640.13119862241446464No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC630.12914864393923864No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC630.12914864393923864No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC630.12914864393923864No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC630.12914864393923864No Hit
GTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC620.12709866546401263No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC620.12709866546401263No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC620.12709866546401263No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC610.12504868698878663No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC610.12504868698878663No Hit
CCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC610.12504868698878663No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC600.12299870851356061No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC590.1209487300383346No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC580.11889875156310858No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC580.11889875156310858No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC570.11684877308788258No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC570.11684877308788258No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC570.11684877308788258No Hit
TAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC570.11684877308788258No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC570.11684877308788258No Hit
ATGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC570.11684877308788258No Hit
TTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC560.11479879461265656No Hit
CGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC550.11274881613743054No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC550.11274881613743054No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC550.11274881613743054No Hit
AGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC550.11274881613743054No Hit
GTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC550.11274881613743054No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC540.11069883766220455No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC540.11069883766220455No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC540.11069883766220455No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC540.11069883766220455No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC540.11069883766220455No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC540.11069883766220455No Hit
TTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC540.11069883766220455No Hit
CGTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC530.10864885918697854No Hit
ACTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC530.10864885918697854No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC530.10864885918697854No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC530.10864885918697854No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC530.10864885918697854No Hit
GTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC520.10659888071175252No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC520.10659888071175252No Hit
TACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC520.10659888071175252No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC520.10659888071175252No Hit
CGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC520.10659888071175252No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC520.10659888071175252No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC510.10454890223652652No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC510.10454890223652652No Hit
GGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC510.10454890223652652No Hit
AGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC510.10454890223652652No Hit
TTGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC510.10454890223652652No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC510.10454890223652652No Hit
CAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC510.10454890223652652No Hit
ACTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC510.10454890223652652No Hit
CAGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC510.10454890223652652No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC510.10454890223652652No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC510.10454890223652652No Hit
TGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC500.1024989237613005No Hit
CGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC500.1024989237613005No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC500.1024989237613005No Hit
TGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC500.1024989237613005No Hit
TGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC500.1024989237613005No Hit
AATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC500.1024989237613005No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC500.1024989237613005No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC500.1024989237613005No Hit
GGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC500.1024989237613005No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC500.1024989237613005No Hit
AATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC500.1024989237613005No Hit
GTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC500.1024989237613005No Hit
CGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC500.1024989237613005No Hit
AGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC490.1004489452860745No Hit
CGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC490.1004489452860745No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC490.1004489452860745No Hit
CCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC490.1004489452860745No Hit
GCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC490.1004489452860745No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC490.1004489452860745No Hit
GGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC490.1004489452860745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAC1700.0295.000033
GCTCCTA850.0295.000032
CGTTCCT108.440226E-4295.01
AGCCTAC1850.0295.03
TTGTCCT108.440226E-4295.01
TGGTCCT255.2386895E-10295.01
AATCCTA750.0295.02
CCTACGA206.124719E-8295.05
GTCACCT206.124719E-8295.01
CCTACCT108.440226E-4295.01
ATCTCCT206.124719E-8295.01
AGCTCCT350.0295.01
TATATGA108.440226E-4295.0295
CTCCCTA750.0295.02
CGCCCTA500.0295.02
AAATCCT450.0295.01
TCCTAGG108.440226E-4295.04
CGGGGGC350.0295.09
AGGACCT255.2386895E-10295.01
GGTCCTA750.0295.02
Produced by FastQC (version 0.11.5)