FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63045
Sequences flagged as poor quality0
Sequence length301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8711.381552859068919No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8571.359346498532794No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8051.276865730827187No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7801.2372115155841066No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7291.1563169164882228No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6921.0976286779284639No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6651.0548021254659372No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6050.9596320088825442No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5880.9326671425172496No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5390.8549448806408122No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5120.8121183281782853No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4530.7185343802046157No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4490.7121897057657229No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4170.66143231025458No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4070.6455706241573479No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4010.6360536124990087No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3900.6186057577920533No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3890.6170195891823301No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3770.5979855658656514No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3730.5916408914267587No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3680.5837100483781426No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3680.5837100483781426No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3270.5186771353794908No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3240.5139186295503212No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3240.5139186295503212No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3230.512332460940598No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3150.4996431120628123No Hit
GGACTACACGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3030.4806090887461337No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3030.4806090887461337No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2930.4647474026489015No Hit
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2870.4552303909905623No Hit
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2840.45047188516139264No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2830.4488857165516695No Hit
GGACTACTCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2830.4488857165516695No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2820.4472995479419462No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2730.4330240304544373No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2680.42509318740582125No Hit
GGACTACTCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2660.4219208501863748No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2510.3981283210405266No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2500.3965421524308034No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2500.3965421524308034No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2430.3854389721627409No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2390.37909429772384806No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2350.3727496232849552No Hit
GGACTACAAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2290.3632326116266159No Hit
GGACTACCCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2230.3537155999682766No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2200.34895709413910697No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2190.3473709255293838No Hit
GGACTACTAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2150.34102625109049095No Hit
GGACTACACGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2140.33944008248076774No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2040.3235783963835356No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2010.31881989055436594No Hit
GGACTACTGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1990.3156475533349195No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1950.30930287889602665No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1950.30930287889602665No Hit
GGACTACCCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1930.3061305416765802No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1920.304544373066857No Hit
GGACTACACGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1910.3029582044571338No Hit
GGACTACTAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1850.29344119279879455No Hit
GGACTACTCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1840.2918550241890713No Hit
GGACTACTGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1820.28868268696962485No Hit
GGACTACAAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1780.282338012530732No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1740.2759933380918391No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1680.2664763264334999No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1670.2648901578237767No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1640.260131651994607No Hit
GGACTACCAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1640.260131651994607No Hit
GGACTACCCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1630.2585454833848838No Hit
GGACTACTAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1590.25220080894599095No Hit
GGACTACCCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1570.24902847172654452No Hit
GGACTACCGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1560.24744230311682133No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1550.24585613450709812No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1550.24585613450709812No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1540.24426996589737487No Hit
GGACTACTCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1530.24268379728765166No Hit
GGACTACCGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1510.23951146006820526No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1490.2363391228487588No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1450.22999444840986596No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1440.22840827980014278No Hit
GGACTACAGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1420.22523594258069632No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1380.21889126814180349No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1370.21730509953208024No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1320.20937425648346417No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1320.20937425648346417No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1310.20778808787374098No Hit
GGACTACAGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1300.20620191926401776No Hit
GGACTACCAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1300.20620191926401776No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1220.19351257038623207No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1220.19351257038623207No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1200.19034023316678564No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1190.18875406455706242No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1170.185581727337616No Hit
GGACTACTGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1150.18240939011816956No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1100.17447854706955349No Hit
GGACTACACGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1080.17130620985010706No Hit
GGACTACCGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1070.16972004124038387No Hit
GGACTACCAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1070.16972004124038387No Hit
GGACTACTCGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1060.16813387263066062No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1050.16654770402093744No Hit
GGACTACTGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1010.16020302958204458No Hit
GGACTACAAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1010.16020302958204458No Hit
GGACTACACGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC980.15544452375287493No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA980.15544452375287493No Hit
GGACTACAAGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC980.15544452375287493No Hit
GGACTACAAGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC970.15385835514315171No Hit
GGACTACCAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT960.1522721865334285No Hit
GGACTACAGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT940.14909984931398207No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA940.14909984931398207No Hit
GGACTACACGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC930.14751368070425888No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC900.14275517487508924No Hit
GGACTACTCGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC900.14275517487508924No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA900.14275517487508924No Hit
GGACTACTAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA900.14275517487508924No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA880.1395828376556428No Hit
GGACTACACGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA880.1395828376556428No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA850.13482433182647316No Hit
GGACTACTAGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC850.13482433182647316No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA840.13323816321674994No Hit
GGACTACCCGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA840.13323816321674994No Hit
GGACTACCCGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC830.13165199460702673No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA820.1300658259973035No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC820.1300658259973035No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA810.1284796573875803No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA800.12689348877785708No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA800.12689348877785708No Hit
GGACTACTCGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA800.12689348877785708No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC780.12372115155841067No Hit
GGACTACAGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT780.12372115155841067No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA780.12372115155841067No Hit
GGACTACTCGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA770.12213498294868744No Hit
GGACTACTAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA770.12213498294868744No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC770.12213498294868744No Hit
GGACTACTAGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC770.12213498294868744No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA770.12213498294868744No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC750.11896264572924102No Hit
GGACTACAAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA740.11737647711951782No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA730.11579030850979459No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC720.11420413990007139No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC720.11420413990007139No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA720.11420413990007139No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA720.11420413990007139No Hit
GGACTACCGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT710.11261797129034816No Hit
GGACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC710.11261797129034816No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA690.10944563407090174No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA690.10944563407090174No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA680.10785946546117851No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA670.10627329685145531No Hit
GGACTACTGGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC670.10627329685145531No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA670.10627329685145531No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA660.10468712824173208No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA650.10310095963200888No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA640.10151479102228567No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA4250.0295.08
CGTTAGC750.0295.0295
GGTTAGC108.446082E-4295.0295
GGACTAC58750.0294.497861
CTACAAG10700.0293.62154
TACAAGG10700.0293.62155
ACCCGGG5800.0292.456886
ACAAGGG10750.0292.255836
ACTACCG4850.0291.958773
CTACCGG4850.0291.958774
ACACGGG8750.0291.628576
CTACTCG8650.0291.58964
CGGGTAT12000.0291.31259
GACTACA24150.0290.11392
GACTACC14950.0290.06692
TACACGG8850.0290.000035
CTACCCG5800.0289.91384
TACTCGG8700.0289.91385
CAAGGGT10750.0289.511637
CGGGGTA2400.0288.854168
Produced by FastQC (version 0.11.5)