FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005491280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97661
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA7770.7956093015635719No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA7030.7198369871289461No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA4550.465897338753443No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA4080.417771679585505No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA4000.40958007802500485No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3360.34404726554100407No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3290.3368796141755665No Hit
GGACTACTCGGGTTTCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA3250.3327838133953165No Hit
GGACTACCCGGGTTTCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA3090.31640061027431626No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3030.3102569091039412No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2630.2692989013014407No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCTAGCTTTCGCGTCTCA2580.26417915032612815No Hit
GGACTACTCGGGTTTCTAATGCCGTCTTCTGTTGCTGCTGCCTCCCGTAG2250.23038879388906525No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2190.22424509271869014No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2000.20479003901250242No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCTAGCTTTCGCGTCTCA1860.19045473628162726No Hit
GGACTACTAGGGTTTCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1820.18635893550137722No Hit
GGACTACTGGGGTTTCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1770.18123918452606463No Hit
GGACTACCCGGGTTTCTAATGCCGTCTTCTGTTGCTGCTGCCTCCCGTAG1760.18021523433100214No Hit
GGACTACTCGGGTTTCTATGCCGTCTTCTGCTTGCTGCTGCCTCCCGTAG1680.17202363277050203No Hit
GGACTACTCGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1660.16997573238037703No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1620.16587993160012698No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1560.1597362304297519No Hit
GGACTACTCGGGTTTCTAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA1550.1587122802346894Illumina Single End PCR Primer 1 (100% over 32bp)
GGACTACTCGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCGTCTCA1530.15666437984456436No Hit
GGACTACCCGGGTTTCTATGCCGTCTTCTGCTTGCTGCTGCCTCCCGTAG1480.1515446288692518No Hit
GGACTACTCGGGTTTCTAATCCCATTCGCTCCCCTAGCTTTCGTCCCTCA1460.14949672847912676No Hit
GGACTACCCGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1450.14847277828406427No Hit
GGACTACACGGGTTTCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1420.14540092769887672No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCTAGCTTTCGCGTCTCA1320.1351614257482516No Hit
GGACTACTAGGGTTTCTAATGCCGTCTTCTGTTGCTGCTGCCTCCCGTAG1310.13413747555318908No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCTAGCTTTCGCGTCTCA1280.13106562496800156No Hit
GGACTACCGGGGTTTCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1280.13106562496800156No Hit
GGACTACCCGGGTTTCTAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA1250.12799377438281404Illumina Single End PCR Primer 1 (100% over 32bp)
GGACTACCCGGGTTTCTAATCCCATTCGCTCCCCTAGCTTTCGTCCCTCA1160.1187782226272514No Hit
GGACTACTAGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1150.11775427243218889No Hit
GGACTACTGGGGTTTCTATGCCGTCTTCTGCTTGCTGCTGCCTCCCGTAG1140.1167303222371264No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCTAGCTTTCGCGTCTCA1120.11468242184700136No Hit
GGACTACTAGGGTTTCTATGCCGTCTTCTGCTTGCTGCTGCCTCCCGTAG1090.11161057126181381No Hit
GGACTACTGGGGTTTCTAATGCCGTCTTCTGTTGCTGCTGCCTCCCGTAG1080.11058662106675132No Hit
GGACTACTCGGGTATCTAATGCCGTCTTCTGTTGCTGCTGCCTCCCGTAG1070.1095626708716888No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1070.1095626708716888No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1050.10751477048156377No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1040.10649082028650125No Hit
GGACTACAAGGGTTTCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1020.10444291989637625No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1010.10341896970131373No Hit
GGACTACCCGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCGTCTCA990.10137106931118871No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA980.10034711911612619No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)