FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72348
Sequences flagged as poor quality0
Sequence length301
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1070.14789627909548295No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1070.14789627909548295No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1070.14789627909548295No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1070.14789627909548295No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1050.14513186266379166No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1030.1423674462321004No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1020.1409852380162548No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1000.1382208215845635No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC980.13545640515287222No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC980.13545640515287222No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC970.1340741969370266No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC960.13269198872118096No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC950.13130978050533532No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC940.12992757228948967No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC930.12854536407364406No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC930.12854536407364406No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC930.12854536407364406No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC920.12716315585779842No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC920.12716315585779842No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC910.1257809476419528No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC900.12439873942610714No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC900.12439873942610714No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC890.12301653121026151No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC890.12301653121026151No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC890.12301653121026151No Hit
ATGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC890.12301653121026151No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.12163432299441587No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.12163432299441587No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.12163432299441587No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.12163432299441587No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC870.12025211477857026No Hit
CTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC870.12025211477857026No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC870.12025211477857026No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC870.12025211477857026No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.11886990656272461No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.11886990656272461No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.11886990656272461No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.11886990656272461No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC850.11748769834687897No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.11610549013103333No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.1147232819151877No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.1147232819151877No Hit
GTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.1147232819151877No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.1147232819151877No Hit
ATCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.11334107369934207No Hit
CAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.11195886548349644No Hit
CATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.11195886548349644No Hit
AGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.11195886548349644No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.11195886548349644No Hit
CATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.11195886548349644No Hit
TCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.11195886548349644No Hit
TTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.1105766572676508No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.1105766572676508No Hit
CTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.1105766572676508No Hit
TGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC790.10919444905180516No Hit
GGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC790.10919444905180516No Hit
ACGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC790.10919444905180516No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC790.10919444905180516No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.10781224083595953No Hit
TGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.10781224083595953No Hit
GGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.10781224083595953No Hit
TCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.10781224083595953No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.10781224083595953No Hit
GGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.1064300326201139No Hit
ACGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.1064300326201139No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.1064300326201139No Hit
GGTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC760.10504782440426827No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC760.10504782440426827No Hit
CTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC760.10504782440426827No Hit
GCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC760.10504782440426827No Hit
ACCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC760.10504782440426827No Hit
ACCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC760.10504782440426827No Hit
CCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC760.10504782440426827No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC760.10504782440426827No Hit
ATCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC750.10366561618842263No Hit
TTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC750.10366561618842263No Hit
AGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC750.10366561618842263No Hit
CCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC750.10366561618842263No Hit
TATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC740.10228340797257698No Hit
AGGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC740.10228340797257698No Hit
AGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC740.10228340797257698No Hit
CGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC740.10228340797257698No Hit
CACGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC740.10228340797257698No Hit
TGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.10090119975673134No Hit
CGACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.10090119975673134No Hit
CCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.10090119975673134No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.10090119975673134No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.10090119975673134No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.10090119975673134No Hit
AGTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.10090119975673134No Hit
GCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.10090119975673134No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.10090119975673134No Hit
GAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.10090119975673134No Hit
CGGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.10090119975673134No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.10090119975673134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCCT350.0295.000031
ATTACCT350.0295.000031
CGTTCCT350.0295.000031
GTCACCT350.0295.000031
CGATCCT350.0295.000031
CCGCCCT350.0295.000031
TGCACCT350.0295.000031
TGATCCT255.2568794E-10295.01
TCGCCTA1000.0295.02
GTTGATT255.2568794E-10295.0295
GTTGAGA108.4486575E-4295.0295
GTGCAGT108.4486575E-4295.0295
TTGGCCT400.0295.01
GTCGCCT157.212391E-6295.01
TGCCCCT206.145092E-8295.01
AGATCCT303.6379788E-12295.01
GCGACCT303.6379788E-12295.01
TCACCCT400.0295.01
GTACAGG255.2568794E-10295.0295
GAGTCCT108.4486575E-4295.01
Produced by FastQC (version 0.11.5)