FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82582
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC860.10413891647090166No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCCT350.0295.000031
ACGACCT350.0295.000031
TGATCCT500.0295.01
CGCGCCT255.275069E-10295.01
GCGCCTA1150.0295.02
GCGCCCT255.275069E-10295.01
ACGGGGC157.216093E-6295.08
TTCCCTA1350.0295.02
ACCTAGG108.450827E-4295.04
CGCTCCT650.0295.01
GTTCCCT303.6379788E-12295.01
GGCGCCT303.6379788E-12295.01
GATTCCT255.275069E-10295.01
CGATCCT600.0295.01
CCCGCCT206.150367E-8295.01
TTCCTCG157.216093E-6295.03
TATCCTA1450.0295.02
TGCCGGA108.450827E-4295.0295
CCCTACG16650.0291.456484
CCTACGG75550.0290.50965
Produced by FastQC (version 0.11.5)