FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89775
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1190.13255360623781678No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1090.1214146477304372No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1080.12030075187969924No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1030.11473127262600948No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1020.11361737677527152No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1010.11250348092453355No Hit
CCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1000.1113895850737956No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1000.1113895850737956No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC990.11027568922305764No Hit
CTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC980.10916179337231968No Hit
TCGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC980.10916179337231968No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC980.10916179337231968No Hit
TCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC970.10804789752158174No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC960.10693400167084378No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC960.10693400167084378No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC940.10470620996936787No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC940.10470620996936787No Hit
AAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC940.10470620996936787No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC930.1035923141186299No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC930.1035923141186299No Hit
TATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC920.10247841826789195No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC920.10247841826789195No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC920.10247841826789195No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC900.10025062656641603No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCCT255.275069E-10294.996121
CAGCCTA1000.0294.996122
CACGCCT500.0294.996121
ACCTCCT500.0294.996121
AATGCCT255.275069E-10294.996121
GTAGCCT255.275069E-10294.996121
TACTCCT500.0294.996121
GGGCCCT255.275069E-10294.996121
GACGCCT255.275069E-10294.996121
CGCCCTA1150.0294.996122
TGATCCT303.6379788E-12294.99611
GCCCCTA700.0294.99612
GTCGCCT350.0294.99611
TGCCCCT206.153823E-8294.99611
AGATCCT206.153823E-8294.99611
ACGCCTA1400.0294.99612
GTACAGT108.452388E-4294.9961295
GAGTCCT350.0294.99611
CCGGCCT400.0294.99611
CGCATAC157.2185703E-6294.9961295
Produced by FastQC (version 0.11.5)