FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41726
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT770.1845372190001438No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT740.1773474572209174No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT720.17255428270143316No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT720.17255428270143316No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT720.17255428270143316No Hit
CCATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT700.1677611081819489No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT690.1653645209222068No Hit
TGATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT680.16296793366246465No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT660.15817475914298038No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT640.15338158462349616No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT630.150984997363754No Hit
TCTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT630.150984997363754No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT630.150984997363754No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT620.1485884101040119No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT620.1485884101040119No Hit
TATTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT620.1485884101040119No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT610.14619182284426976No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT610.14619182284426976No Hit
GTAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT610.14619182284426976No Hit
AACACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT610.14619182284426976No Hit
AACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT610.14619182284426976No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT610.14619182284426976No Hit
AAAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT600.14379523558452764No Hit
CAGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT600.14379523558452764No Hit
GTTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT590.1413986483247855No Hit
GCATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT590.1413986483247855No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT590.1413986483247855No Hit
TTCCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT590.1413986483247855No Hit
GTACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT590.1413986483247855No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT590.1413986483247855No Hit
CGGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT590.1413986483247855No Hit
TTACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT580.13900206106504337No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT580.13900206106504337No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT580.13900206106504337No Hit
GTCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT570.13660547380530125No Hit
TCGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT570.13660547380530125No Hit
ATTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT570.13660547380530125No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT570.13660547380530125No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT570.13660547380530125No Hit
GTGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT560.13420888654555913No Hit
CGTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT560.13420888654555913No Hit
CGGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT560.13420888654555913No Hit
TCACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT560.13420888654555913No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT560.13420888654555913No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT560.13420888654555913No Hit
TAACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT550.131812299285817No Hit
TCCGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT550.131812299285817No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT550.131812299285817No Hit
CTTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT550.131812299285817No Hit
TGACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT550.131812299285817No Hit
TGGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT550.131812299285817No Hit
ATGCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT550.131812299285817No Hit
CATTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT530.12701912476633276No Hit
CCACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT530.12701912476633276No Hit
CCCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT530.12701912476633276No Hit
GTGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT530.12701912476633276No Hit
TGAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT520.12462253750659062No Hit
CAGCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT520.12462253750659062No Hit
GGGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT520.12462253750659062No Hit
ACCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT520.12462253750659062No Hit
CTCGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT520.12462253750659062No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT520.12462253750659062No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT520.12462253750659062No Hit
TATCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT510.12222595024684849No Hit
ACCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT510.12222595024684849No Hit
ACTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT510.12222595024684849No Hit
GAAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT510.12222595024684849No Hit
CAACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT510.12222595024684849No Hit
ATGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT510.12222595024684849No Hit
GTTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT510.12222595024684849No Hit
TATACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT500.11982936298710635No Hit
AGCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT500.11982936298710635No Hit
TAGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT500.11982936298710635No Hit
TGTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT490.11743277572736424No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT490.11743277572736424No Hit
TCCCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT490.11743277572736424No Hit
AGCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT490.11743277572736424No Hit
GCCCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT490.11743277572736424No Hit
TCGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT490.11743277572736424No Hit
CACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT490.11743277572736424No Hit
TTGGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT490.11743277572736424No Hit
TTCGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT490.11743277572736424No Hit
GAGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT490.11743277572736424No Hit
CGCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT490.11743277572736424No Hit
ATGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT480.1150361884676221No Hit
TTTCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT480.1150361884676221No Hit
GGCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT480.1150361884676221No Hit
ACCCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT480.1150361884676221No Hit
AAACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT480.1150361884676221No Hit
CGATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT480.1150361884676221No Hit
CCTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT480.1150361884676221No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT480.1150361884676221No Hit
ATCCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT480.1150361884676221No Hit
ATGGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT470.11263960120787997No Hit
TAGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT470.11263960120787997No Hit
TACGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT470.11263960120787997No Hit
GTCGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT470.11263960120787997No Hit
TACCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT470.11263960120787997No Hit
CTCCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT470.11263960120787997No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT470.11263960120787997No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT470.11263960120787997No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT470.11263960120787997No Hit
TTAGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT460.11024301394813785No Hit
ATTGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT460.11024301394813785No Hit
AGAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT460.11024301394813785No Hit
CAGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT460.11024301394813785No Hit
CCGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT460.11024301394813785No Hit
AATCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT460.11024301394813785No Hit
TCCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT460.11024301394813785No Hit
CCTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT460.11024301394813785No Hit
ACGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT450.10784642668839574No Hit
AGTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT450.10784642668839574No Hit
GGAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT450.10784642668839574No Hit
GAATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT450.10784642668839574No Hit
CTGGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT450.10784642668839574No Hit
CGTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT450.10784642668839574No Hit
ACGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT450.10784642668839574No Hit
TCGCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT450.10784642668839574No Hit
CTGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT450.10784642668839574No Hit
TCTCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT450.10784642668839574No Hit
TCGGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT450.10784642668839574No Hit
ACTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT450.10784642668839574No Hit
CCAGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT440.1054498394286536No Hit
CATCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT440.1054498394286536No Hit
GGATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT440.1054498394286536No Hit
CCCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT440.1054498394286536No Hit
TTTGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT440.1054498394286536No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT440.1054498394286536No Hit
CATACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT440.1054498394286536No Hit
AAAGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT440.1054498394286536No Hit
GTCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT440.1054498394286536No Hit
CTGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT440.1054498394286536No Hit
TATGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT430.10305325216891147No Hit
AGATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT430.10305325216891147No Hit
GCCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT430.10305325216891147No Hit
AATACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT430.10305325216891147No Hit
TGCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT430.10305325216891147No Hit
AAGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT430.10305325216891147No Hit
AATTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT430.10305325216891147No Hit
CGGCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT430.10305325216891147No Hit
ATACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT430.10305325216891147No Hit
TAGCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT430.10305325216891147No Hit
CAAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT430.10305325216891147No Hit
GGTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT430.10305325216891147No Hit
AGGCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT430.10305325216891147No Hit
ATCGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT430.10305325216891147No Hit
CTAGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT420.10065666490916933No Hit
CTTCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT420.10065666490916933No Hit
AAGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT420.10065666490916933No Hit
ACGCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT420.10065666490916933No Hit
AAGCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT420.10065666490916933No Hit
GTAGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT420.10065666490916933No Hit
GCCGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT420.10065666490916933No Hit
TGTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT420.10065666490916933No Hit
ACAGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT420.10065666490916933No Hit
AGGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT420.10065666490916933No Hit
GGGCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT420.10065666490916933No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCTAC1900.0295.000033
CAGCCCT206.114169E-8295.01
CGTTCCT157.1905324E-6295.01
ATCCCCT108.435851E-4295.01
AGCCCCT206.114169E-8295.01
ACAGCCT303.6379788E-12295.01
TTGTCCT303.6379788E-12295.01
TGGTCCT157.1905324E-6295.01
ACGCCTA600.0295.02
TGCACCT108.435851E-4295.01
GCCTCCT108.435851E-4295.01
CCTACGA108.435851E-4295.05
GGCACCT157.1905324E-6295.01
GTGCAGT108.435851E-4295.0295
ATCTCCT255.2204996E-10295.01
AGCTCCT303.6379788E-12295.01
TGACCTA500.0295.02
CTCCCTA550.0295.02
CGCCCTA450.0295.02
TTCGCCT255.2204996E-10295.01
Produced by FastQC (version 0.11.5)