FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83406
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC880.10550799702659282No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC870.10430904251492697No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC870.10430904251492697No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC850.10191113349159533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCCTA950.0295.000032
ACCCCTA950.0295.000032
TTGCCTA950.0295.000032
CAGCCCT255.275069E-10295.01
GCCCTAG157.2163584E-6295.03
GCCCTAC4000.0295.03
CGTTCCT303.6379788E-12295.01
ATCCCCT255.275069E-10295.01
AAACCTA1300.0295.02
GTTGATT157.2163584E-6295.0295
ACGCCTA1050.0295.02
TGCACCT206.150731E-8295.01
CCTACGA206.150731E-8295.05
GTCACCT500.0295.01
CCTACCT400.0295.01
ATCTCCT400.0295.01
AGCTCCT350.0295.01
CACACCT206.150731E-8295.01
GCCCACG108.450983E-4295.03
CTCCCTA850.0295.02
Produced by FastQC (version 0.11.5)