FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58188
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC940.1615453358080704No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC900.15467106619921633No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC890.15295249879700282No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC890.15295249879700282No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC870.14951536399257578No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC850.14607822918814875No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC830.14264109438372172No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC820.14092252698150823No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC820.14092252698150823No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.13920395957929468No Hit
TCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.13920395957929468No Hit
GTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.13920395957929468No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.1374853921770812No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC790.13576682477486765No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC790.13576682477486765No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.13232968997044064No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.13232968997044064No Hit
TCGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.13061112256822713No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.13061112256822713No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.13061112256822713No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC750.1288925551660136No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC750.1288925551660136No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC750.1288925551660136No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.1271739877638001No Hit
TAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.12545542036158658No Hit
TGCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.12545542036158658No Hit
TCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.12545542036158658No Hit
CTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.12545542036158658No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.12545542036158658No Hit
CAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.12373685295937306No Hit
ACACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.12373685295937306No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.12373685295937306No Hit
TTAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.12201828555715956No Hit
GGATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.12029971815494603No Hit
AACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.12029971815494603No Hit
CAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.12029971815494603No Hit
ACCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.12029971815494603No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.12029971815494603No Hit
TATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.12029971815494603No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.11858115075273253No Hit
CGGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.11858115075273253No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.11858115075273253No Hit
TCTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.11858115075273253No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.11858115075273253No Hit
CCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.11858115075273253No Hit
CCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.11858115075273253No Hit
AAGCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.11686258335051902No Hit
GTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.11686258335051902No Hit
AGAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.11686258335051902No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.11686258335051902No Hit
AATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.11514401594830549No Hit
CACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.11514401594830549No Hit
GCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.11514401594830549No Hit
GTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.11514401594830549No Hit
AGCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.11342544854609199No Hit
TTCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.11342544854609199No Hit
GAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.11342544854609199No Hit
CGCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.11170688114387846No Hit
CCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.11170688114387846No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.11170688114387846No Hit
CATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.11170688114387846No Hit
TCGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.11170688114387846No Hit
CTAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10998831374166496No Hit
GAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10826974633945143No Hit
ACTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10826974633945143No Hit
TTTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10826974633945143No Hit
TCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10826974633945143No Hit
AACGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10826974633945143No Hit
TGATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10826974633945143No Hit
ATTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10826974633945143No Hit
TAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10826974633945143No Hit
GCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.10655117893723792No Hit
AGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.10655117893723792No Hit
TTGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.10655117893723792No Hit
CCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.10655117893723792No Hit
CCAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.10655117893723792No Hit
CTTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.10655117893723792No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.10655117893723792No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC610.10483261153502439No Hit
CGGCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC610.10483261153502439No Hit
TCCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC610.10483261153502439No Hit
TTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC610.10483261153502439No Hit
CCCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC610.10483261153502439No Hit
GGTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC610.10483261153502439No Hit
GTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC600.10311404413281089No Hit
TACGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC600.10311404413281089No Hit
TCTGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC600.10311404413281089No Hit
ACCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC600.10311404413281089No Hit
TGTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC600.10311404413281089No Hit
CCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC600.10311404413281089No Hit
ATACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC590.10139547673059739No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC590.10139547673059739No Hit
CCGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC590.10139547673059739No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCTA550.0295.000032
GGGCCTA550.0295.000032
CGTTCCT255.2568794E-10295.01
ATCCCCT108.44441E-4295.01
AGCCTAC2550.0295.03
GTTGATT108.44441E-4295.0295
TGGTCCT108.44441E-4295.01
AATCCTA850.0295.02
AACGCCT157.205137E-6295.01
TCCTCCT303.6379788E-12295.01
TTCACCT350.0295.01
TGCACCT157.205137E-6295.01
GGGTCCT206.1349056E-8295.01
GCCTCCT303.6379788E-12295.01
CCTACCT255.2568794E-10295.01
ATCTCCT255.2568794E-10295.01
AGCTCCT206.1349056E-8295.01
CTCCCTA1000.0295.02
CGCCCTA600.0295.02
AAATCCT255.2568794E-10295.01
Produced by FastQC (version 0.11.5)