FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73169
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1140.15580368735393405No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC960.13120310514015499No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC930.12710300810452516No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC920.12573630909264855No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC920.12573630909264855No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC920.12573630909264855No Hit
GTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC920.12573630909264855No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC900.12300291106889528No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC880.12026951304514207No Hit
ATTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC870.11890281403326546No Hit
GCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC870.11890281403326546No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC870.11890281403326546No Hit
AACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC860.11753611502138885No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC860.11753611502138885No Hit
TCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC850.11616941600951222No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC830.113436017985759No Hit
ATACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC830.113436017985759No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC830.113436017985759No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC830.113436017985759No Hit
CAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC830.113436017985759No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC820.11206931897388239No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC820.11206931897388239No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC810.11070261996200575No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC810.11070261996200575No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC800.10933592095012915No Hit
TTTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC800.10933592095012915No Hit
TCGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC800.10933592095012915No Hit
CTCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC790.10796922193825254No Hit
CAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC790.10796922193825254No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC790.10796922193825254No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC780.10660252292637593No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC780.10660252292637593No Hit
AACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC780.10660252292637593No Hit
CACCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC780.10660252292637593No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC770.1052358239144993No Hit
CCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC770.1052358239144993No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC770.1052358239144993No Hit
CACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC760.10386912490262269No Hit
CGATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC760.10386912490262269No Hit
TATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC760.10386912490262269No Hit
TATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC760.10386912490262269No Hit
CATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC750.10250242589074608No Hit
TGAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC750.10250242589074608No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC750.10250242589074608No Hit
TAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC750.10250242589074608No Hit
CCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC750.10250242589074608No Hit
TTCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC740.10113572687886947No Hit
TTGCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC740.10113572687886947No Hit
ACACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC740.10113572687886947No Hit
ACCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC740.10113572687886947No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCCT450.0295.200441
TGATCCT400.0295.20041
TCGCCTA1300.0295.20042
GCCCCCT206.124901E-8295.20041
TTGGCCT400.0295.20041
TGCCCCT400.0295.20041
CGTGCCT108.431683E-4295.20041
GATCCCT303.6379788E-12295.20041
CACGCCT157.19319E-6295.20041
CGCGCCT255.2386895E-10295.20041
GGATCCT206.124901E-8295.20041
TGAACCT206.124901E-8295.20041
GCGCCCT255.2386895E-10295.20041
CGTTCCT255.2386895E-10295.20041
GACCCCT206.124901E-8295.20041
ACCTCCT400.0295.20041
GTAGCCT108.431683E-4295.20041
TGCGCCT108.431683E-4295.20041
CGGGCCT157.19319E-6295.20041
GGAACCT206.124901E-8295.20041
Produced by FastQC (version 0.11.5)