FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98534
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCCTACGGGAGGCAGCAGGACTAGGGGGTTGGGGACAGATTTTATTG1150.11671098301094039No Hit
ATATCCTACGGGAGGCAGCAGGACTAGGGGGTTGGGGACAGATTTTATTG1090.1106217143321087No Hit
TCAACCTACGGGAGGCAGCAGGACTAGGGGGTTGGGGACAGATTTTATTG1070.10859195810583148No Hit
ATAACCTACGGGAGGCAGCAGGACTAGGGGGTTGGGGACAGATTTTATTG1070.10859195810583148No Hit
TTACCCTACGGGAGGCAGCAGGACTAGGGGGTTGGGGACAGATTTTATTG1060.10757707999269286No Hit
CTCACCTACGGGAGGCAGCAGGACTAGGGGGTTGGGGACAGATTTTATTG1050.10656220187955426No Hit
CTTTCCTACGGGAGGCAGCAGGACTAGGGGGTTGGGGACAGATTTTATTG1050.10656220187955426No Hit
TCATCCTACGGGAGGCAGCAGGACTAGGGGGTTGGGGACAGATTTTATTG1050.10656220187955426No Hit
GTAACCTACGGGAGGCAGCAGGACTAGGGGGTTGGGGACAGATTTTATTG1040.10554732376641565No Hit
TACTCCTACGGGAGGCAGCAGGACTAGGGGGTTGGGGACAGATTTTATTG1040.10554732376641565No Hit
TATTCCTACGGGAGGCAGCAGGACTAGGGGGTTGGGGACAGATTTTATTG1040.10554732376641565No Hit
CTTACCTACGGGAGGCAGCAGGACTAGGGGGTTGGGGACAGATTTTATTG1030.10453244565327705No Hit
TTCTCCTACGGGAGGCAGCAGGACTAGGGGGTTGGGGACAGATTTTATTG1020.10351756754013842No Hit
TTCACCTACGGGAGGCAGCAGGACTAGGGGGTTGGGGACAGATTTTATTG1020.10351756754013842No Hit
GTATCCTACGGGAGGCAGCAGGACTAGGGGGTTGGGGACAGATTTTATTG1010.10250268942699982No Hit
CTACCCTACGGGAGGCAGCAGGACTAGGGGGTTGGGGACAGATTTTATTG1000.10148781131386121No Hit
TCCACCTACGGGAGGCAGCAGGACTAGGGGGTTGGGGACAGATTTTATTG1000.10148781131386121No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCCTA1100.0295.000032
CCTACGT108.4533024E-4295.05
CCTACGC157.220322E-6295.05
CGTTCCT400.0295.01
GCACCCT500.0295.01
AAGCCTA1350.0295.02
GGGACCT400.0295.01
CTCGGGA400.0295.06
TACGAGG157.220322E-6295.07
CTCGCCT206.1563696E-8295.01
TTACGGG108.4533024E-4295.06
GCTACCT255.275069E-10295.01
CTCCTCG108.4533024E-4295.03
CTTACGG108.4533024E-4295.05
CGGACCT400.0295.01
GCCTACG18150.0290.93664
CCTACGG94100.0289.984075
CCCTACG22300.0289.708534
CTACGGG92250.0289.24396
TACGGGA91300.0289.1847
Produced by FastQC (version 0.11.5)