FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98534
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACTAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG20262.056143057218828No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG18321.8592567032699372No Hit
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG17321.757768891956076No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG14811.5030344855582844No Hit
GGACTACTGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG14441.4654839953721557No Hit
GGACTACTCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG11951.2127793452006415No Hit
GGACTACCGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG11931.210749588974364No Hit
GGACTACCAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG11521.1691395863356813No Hit
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG10171.0321310410619684No Hit
GGACTACCCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG8310.8433637120181867No Hit
GGACTACTCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG8210.8332149308868004No Hit
GGACTACCCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG6740.6840278482554245No Hit
GGACTACCAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG6190.6282095520328008No Hit
GGACTACACGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG6080.6170458927882762No Hit
GGACTACAAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT5430.5510788154342664No Hit
GGACTACCGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5430.5510788154342664No Hit
GGACTACTAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT5260.5338258875109099No Hit
GGACTACAAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT4420.44857612600726654No Hit
GGACTACTAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT4370.4435017354415735No Hit
GGACTACTGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT4330.439442222989019No Hit
GGACTACAGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3630.3684007550693162No Hit
GGACTACCGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3330.3379544116751578No Hit
GGACTACCAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3310.33592465544888056No Hit
GGACTACACGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3130.3176568494123856No Hit
GGACTACTCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2980.3024336777153064No Hit
GGACTACTCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2870.29127001847078166No Hit
GGACTACTAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2790.28315099356567275No Hit
GGACTACAAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2730.2770617248868411No Hit
GGACTACTAGGGTATCTAACTGAAGGGGCGATTTCCCTTCACATGTCCAC2680.271987334321148No Hit
GGACTACAAGGGTATCTAACTGAAGGGGCGATTTCCCTTCACATGTCCAC2380.24154099092698966No Hit
GGACTACCCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2370.24052611281385108No Hit
GGACTACAAGGGTTTCTAACTGAAGGGGCGATTTCCCTTCACATGTCCAC2370.24052611281385108No Hit
GGACTACTAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2370.24052611281385108No Hit
GGACTACTGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2300.23342196602188078No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2290.23240708790874215No Hit
GGACTACCCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2290.23240708790874215No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC2230.22631781922991048No Hit
GGACTACAAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2200.22327318489049466No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC2140.21718391621166297No Hit
GGACTACTGGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2140.21718391621166297No Hit
GGACTACTAGGGTTTCTAACTGAAGGGGCGATTTCCCTTCACATGTCCAC2090.21210952564596994No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2010.203990500740861No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC2000.20297562262772242No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1960.19891611017516797No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1960.19891611017516797No Hit
GGACTACTCGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1870.18978220715692046No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1840.1867375728175046No Hit
GGACTACTGGGGTTTCTAACTGAAGGGGCGATTTCCCTTCACATGTCCAC1830.185722694704366No Hit
GGACTACCGGGGTTTCTAACTGAAGGGGCGATTTCCCTTCACATGTCCAC1820.1847078165912274No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1780.18064830413867294No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1770.1796334260255343No Hit
GGACTACCAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1730.17557391357297988No Hit
GGACTACACGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1710.17354415734670267No Hit
GGACTACTCGGGTATCTAACTGAAGGGGCGATTTCCCTTCACATGTCCAC1690.17151440112042543No Hit
GGACTACCGGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1680.17049952300728682No Hit
GGACTACAAGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1670.16948464489414822No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1670.16948464489414822No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1660.1684697667810096No Hit
GGACTACTAGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1660.1684697667810096No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1610.16339537621531655No Hit
GGACTACCAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1560.15832098564962346No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1540.15629122942334625No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1530.15527635131020764No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1510.15324659508393043No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1490.1512168388576532No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1470.14918708263137598No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1470.14918708263137598No Hit
GGACTACCAGGGTTTCTAACTGAAGGGGCGATTTCCCTTCACATGTCCAC1460.14817220451823737No Hit
GGACTACAAGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1450.14715732640509877No Hit
GGACTACACGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1430.14512757017882152No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1420.1441126920656829No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1390.14106805772626707No Hit
GGACTACTAGGGTATCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG1390.14106805772626707No Hit
GGACTACTAGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1380.14005317961312846No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1380.14005317961312846No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1380.14005317961312846No Hit
GGACTACAAGGGTTTCTAAGTCTGGTCCTTCAACCAAGGGACTGACAACT1340.135993667160574No Hit
GGACTACTAGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1330.1349787890474354No Hit
GGACTACAAGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1320.1339639109342968No Hit
GGACTACAAGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG1290.13091927659488095No Hit
GGACTACTCGGGTTTCTAACTGAAGGGGCGATTTCCCTTCACATGTCCAC1290.13091927659488095No Hit
GGACTACAAGGGTTTCTAATAAGGGTTTGGGGACCTGACATAATCTCATT1270.12888952036860374No Hit
GGACTACTAGGGTATCTAATAAGGGTTTGGGGACCTGACATAATCTCATT1260.1278746422554651No Hit
GGACTACTAGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG1240.1258448860291879No Hit
GGACTACTCGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1240.1258448860291879No Hit
GGACTACACGGGTTTCTAACTGAAGGGGCGATTTCCCTTCACATGTCCAC1220.12381512980291066No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1220.12381512980291066No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1220.12381512980291066No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1210.12280025168977206No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1210.12280025168977206No Hit
GGACTACAAGGGTATCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG1200.12178537357663344No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1200.12178537357663344No Hit
GGACTACTGGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG1200.12178537357663344No Hit
GGACTACCCGGGTATCTAACTGAAGGGGCGATTTCCCTTCACATGTCCAC1180.11975561735035622No Hit
GGACTACTAGGGTATCTAAGTCTGGTCCTTCAACCAAGGGACTGACAACT1180.11975561735035622No Hit
GGACTACCCGGGTTTCTAACTGAAGGGGCGATTTCCCTTCACATGTCCAC1170.11874073923721762No Hit
GGACTACTAGGGTATCTAAGACTCTTGTCAACGTATAAGCCTTCCTGTTG1170.11874073923721762No Hit
GGACTACCGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1150.11671098301094039No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1150.11671098301094039No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1130.11468122678466316No Hit
GGACTACCCGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1120.11366634867152456No Hit
GGACTACAAGGGTATCTAATAAGGGTTTGGGGACCTGACATAATCTCATT1110.11265147055838595No Hit
GGACTACCAGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1100.11163659244524733No Hit
GGACTACAAGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1100.11163659244524733No Hit
GGACTACAAGGGTATCTAAGTCTGGTCCTTCAACCAAGGGACTGACAACT1080.10960683621897009No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1070.10859195810583148No Hit
GGACTACTAGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1070.10859195810583148No Hit
GGACTACTGGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1060.10757707999269286No Hit
GGACTACCGGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1060.10757707999269286No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1050.10656220187955426No Hit
GGACTACAGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1050.10656220187955426No Hit
GGACTACTGGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1040.10554732376641565No Hit
GGACTACTAGGGTTTCTAATAAGGGTTTGGGGACCTGACATAATCTCATT1010.10250268942699982No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTAC95100.0294.37961
ACCCGGG8450.0293.254466
CGGGTAT13250.0292.773569
CCGGGTA4900.0291.98988
TCGGGTT4850.0291.958778
CTACCCG8650.0291.58964
CCCGGGT8500.0291.529427
GGGGTAT3900.0291.217969
GACTACC27750.0290.216222
TACTGGG9200.0290.190225
CTACTCG11050.0289.660644
TACAAGG18800.0289.507975
GGGGTTT15900.0288.50639
TACTCGG11050.0288.32585
CGGGGTA2200.0288.295478
CAAGGGT18950.0287.216377
CACGGGT9400.0287.154247
AGGGTAT21850.0286.224279
ACAAGGG19050.0285.708656
ACACGGG9450.0285.634956
Produced by FastQC (version 0.11.5)