FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78383
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT900.11482081573810647No Hit
ACTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT870.11099345521350293No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT870.11099345521350293No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC870.11099345521350293No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC850.1084418815304339No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.10333873416429583No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.10333873416429583No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.10333873416429583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACCTA950.0295.000032
CTTTCCT550.0295.000031
GGACCTA950.0295.000032
CCGCCTA1100.0295.000032
ACCCCTA950.0295.000032
TAATCCT550.0295.000031
TGATCCT303.6379788E-12295.01
TCGCCTA850.0295.02
TCTGCCT157.214694E-6295.01
GTTTGTC108.450007E-4295.0295
ACAACCT350.0295.01
AATCCCT350.0295.01
GTCGCCT255.275069E-10295.01
CCCCTAC4900.0295.03
AGATCCT255.275069E-10295.01
CGAACCT206.148366E-8295.01
CCCTCCT500.0295.01
CGGCCTA1300.0295.02
GAGTCCT303.6379788E-12295.01
TGGACCT350.0295.01
Produced by FastQC (version 0.11.5)