FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50321
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCTA950.0295.000032
TAGCCTA1100.0295.000032
GAGCCTA550.0295.000032
TGATCCT157.199358E-6295.01
TCGCCTA700.0295.02
TCGCCCT255.2386895E-10295.01
TGCCCCT255.2386895E-10295.01
AGATCCT206.12672E-8295.01
ACGCCTA800.0295.02
GCGACCT206.12672E-8295.01
GTCCTAC3700.0295.03
CGACCTA400.0295.02
CACGCCT255.2386895E-10295.01
TCGGGAT108.4410235E-4295.07
CGCGCCT108.4410235E-4295.01
GCGCCTA450.0295.02
GCGCCCT157.199358E-6295.01
ACGGGGG750.0295.08
ACCTCGG157.199358E-6295.04
GCACCCT206.12672E-8295.01
Produced by FastQC (version 0.11.5)