FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005491326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50321
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC7261.4427376244510244No Hit
GGACTACTGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG6881.3672224319866457No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC6571.3056179328709683No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC6221.2360644661274618No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC6031.1983068698952724No Hit
GGACTACTCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5791.1506130641282963No Hit
GGACTACTGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5391.071123387850003No Hit
GGACTACTAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5391.071123387850003No Hit
GGACTACTCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4520.8982333419447149No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC4480.8902843743168856No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4440.8823354066890563No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC4370.868424713340355No Hit
GGACTACTGGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2560.5087339281810775No Hit
GGACTACCGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2560.5087339281810775No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2530.5027722024602055No Hit
GGACTACTCGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2510.4987977186462908No Hit
GGACTACTAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2250.44712942906540015No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2230.44315494525148547No Hit
GGACTACTGGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2140.4252697680888695No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2130.4232825261819121No Hit
GGACTACCCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2080.41334631664712546No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2040.40539734901929614No Hit
GGACTACACGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1920.38155044613580813No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1880.37360147850797876No Hit
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1870.37161423660102144No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1840.36565251088014944No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1800.3577035432523201No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1800.3577035432523201No Hit
GGACTACTCGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1800.3577035432523201No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1780.35372905943840544No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1780.35372905943840544No Hit
GGACTACCAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1780.35372905943840544No Hit
GGACTACTAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1770.35174181753144806No Hit
GGACTACCGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1710.3398183660897041No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1660.3298821565549174No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1610.31994594702013074No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1550.3080224955783868No Hit
GGACTACAGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1480.2941118022296854No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1470.2921245603227281No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1460.29013731841577073No Hit
GGACTACCAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1430.2841755926948988No Hit
GGACTACCCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1430.2841755926948988No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1420.2821883507879414No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1410.28020110888098404No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1370.2722521412531547No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1350.2682776574392401No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1310.2603286898114108No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1290.25635420599749603No Hit
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1280.25436696409053877No Hit
GGACTACTGGGGTATCTAAAAGGCTAGATAGTAACTGTGGAATTTTGCAA1240.2464179964627094No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1230.24443075455575208No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1200.23846902883488005No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1180.2344945450209654No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1180.2344945450209654No Hit
GGACTACTCGGGTATCTAAAAGGCTAGATAGTAACTGTGGAATTTTGCAA1170.23250730311400805No Hit
GGACTACAGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1170.23250730311400805No Hit
GGACTACTAGGGTATCTAAAAGGCTAGATAGTAACTGTGGAATTTTGCAA1150.2285328193000934No Hit
GGACTACTGGGGTATCTAATACCTCTTGTCCATGTCTTATGATACGGCCA1090.21660936785834942No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1090.21660936785834942No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1060.2106476421374774No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1050.20866040023052004No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1050.20866040023052004No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1050.20866040023052004No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1040.20667315832356273No Hit
GGACTACCCGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1020.20269867450964807No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1000.19872419069573338No Hit
GGACTACTGGGGTTTCTAAAAGGCTAGATAGTAACTGTGGAATTTTGCAA1000.19872419069573338No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC990.19673694878877607No Hit
GGACTACTAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT980.19474970688181872No Hit
GGACTACTAGGGTATCTAATACCTCTTGTCCATGTCTTATGATACGGCCA970.19276246497486138No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA970.19276246497486138No Hit
GGACTACTGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT960.19077522306790407No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA950.18878798116094672No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC910.18083901353311738No Hit
GGACTACCGGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA900.17885177162616006No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC880.17487728781224537No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC870.17289004590528806No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA870.17289004590528806No Hit
GGACTACTCGGGTATCTAATACCTCTTGTCCATGTCTTATGATACGGCCA860.17090280399833072No Hit
GGACTACTCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT860.17090280399833072No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA850.16891556209137337No Hit
GGACTACTGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT850.16891556209137337No Hit
GGACTACCGGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA840.16692832018441606No Hit
GGACTACTGGGGTATCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG810.16096659446354405No Hit
GGACTACAGGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA800.1589793525565867No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC800.1589793525565867No Hit
GGACTACTGGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG800.1589793525565867No Hit
GGACTACTCGGGTTTCTAATACCTCTTGTCCATGTCTTATGATACGGCCA800.1589793525565867No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA780.15500486874267205No Hit
GGACTACTCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT770.1530176268357147No Hit
GGACTACAAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA770.1530176268357147No Hit
GGACTACTGGGGTTTCTAATACCTCTTGTCCATGTCTTATGATACGGCCA770.1530176268357147No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC760.1510303849287574No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA760.1510303849287574No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA760.1510303849287574No Hit
GGACTACACGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA740.1470559011148427No Hit
GGACTACTCGGGTATCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG740.1470559011148427No Hit
GGACTACTCGGGTTTCTAAAAGGCTAGATAGTAACTGTGGAATTTTGCAA730.14506865920788536No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA720.14308141730092805No Hit
GGACTACTAGGGTTTCTAATACCTCTTGTCCATGTCTTATGATACGGCCA700.1391069334870134No Hit
GGACTACTAGGGTTTCTAAAAGGCTAGATAGTAACTGTGGAATTTTGCAA700.1391069334870134No Hit
GGACTACCCGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA690.13711969158005605No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA690.13711969158005605No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA680.1351324496730987No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC670.13314520776614136No Hit
GGACTACTAGGGTATCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG660.13115796585918404No Hit
GGACTACTAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT650.1291707239522267No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC650.1291707239522267No Hit
GGACTACCAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA630.12519624013831204No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC620.1232089982313547No Hit
GGACTACTGGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC610.12122175632439736No Hit
GGACTACTCGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC600.11923451441744003No Hit
GGACTACTAGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC600.11923451441744003No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA600.11923451441744003No Hit
GGACTACACGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA580.11526003060352537No Hit
GGACTACTCGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG580.11526003060352537No Hit
GGACTACTAGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG580.11526003060352537No Hit
GGACTACCAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA580.11526003060352537No Hit
GGACTACTCGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC580.11526003060352537No Hit
GGACTACAGGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA570.11327278869656804No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC560.1112855467896107No Hit
GGACTACTGGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC550.10929830488265335No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA540.10731106297569602No Hit
GGACTACAAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA540.10731106297569602No Hit
GGACTACTGGGGTATCTAACTGAAGGGGCGATTTCCCTTCACATGTCCAC530.1053238210687387No Hit
GGACTACCGGGGTATCTAATACCTCTTGTCCATGTCTTATGATACGGCCA530.1053238210687387No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA530.1053238210687387No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA520.10333657916178136No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC520.10333657916178136No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)