FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78687
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1000.1270857956206235No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC970.12327322175200478No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.12073150583959232No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC930.11818978992717984No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC910.11564807401476737No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC900.11437721605856113No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.1131063581023549No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.1131063581023549No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.1131063581023549No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.1131063581023549No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.1131063581023549No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC870.11056464218994243No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC860.1092937842337362No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC860.1092937842337362No Hit
TGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC860.1092937842337362No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC850.10802292627752996No Hit
ACTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC850.10802292627752996No Hit
ACCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC840.10675206832132372No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC840.10675206832132372No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC830.10548121036511748No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC830.10548121036511748No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC830.10548121036511748No Hit
CCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC830.10548121036511748No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC830.10548121036511748No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.10421035240891126No Hit
TTCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.10421035240891126No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC810.10293949445270502No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC810.10293949445270502No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC810.10293949445270502No Hit
AGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC800.10166863649649878No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC800.10166863649649878No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC790.10039777854029255No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC790.10039777854029255No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC790.10039777854029255No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC790.10039777854029255No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCCT350.0295.01
ATCCCCT450.0295.01
AGCCTAC4000.0295.03
TTGTCCT350.0295.01
TGCACCT157.2147977E-6295.01
GGGTCCT450.0295.01
GCCTCCT303.6379788E-12295.01
GTCACCT303.6379788E-12295.01
CCTACCT600.0295.01
ATCTCCT550.0295.01
AGCTCCT303.6379788E-12295.01
CACACCT350.0295.01
TTACCTA1400.0295.02
TGACCTA1400.0295.02
CGCCCTA1300.0295.02
CGCCTAC4800.0295.03
TGTCCCT303.6379788E-12295.01
AGGACCT255.275069E-10295.01
GTCGCCT450.0295.01
TAAGCCT303.6379788E-12295.01
Produced by FastQC (version 0.11.5)