FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61581
Sequences flagged as poor quality0
Sequence length301
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAG108.44561E-4295.08
ATCCTCG108.44561E-4295.03
ACGCCTA1050.0295.02
CGCGCCT255.2568794E-10295.01
GGATCCT255.2568794E-10295.01
CCTACGC108.44561E-4295.05
GCGCCTA1000.0295.02
GCGCCCT255.2568794E-10295.01
TTCCCTA1050.0295.02
AAGCCTA750.0295.02
CGCTCCT206.137816E-8295.01
AACGCCT255.2568794E-10295.01
GGAACCT206.137816E-8295.01
GAGCCTA450.0295.02
TTGCCTA850.0295.02
GACGCCT206.137816E-8295.01
GATTCCT350.0295.01
TTCGCCT206.137816E-8295.01
ACGAGGC108.44561E-4295.08
CGATCCT108.44561E-4295.01
Produced by FastQC (version 0.11.5)