FastQCFastQC Report
Wed 8 May 2024
EGAF00005491335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63491
Total Bases19.1 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC870.13702729520719473No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC790.12442708415365958No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC780.12285205777196768No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC750.11812697862689199No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC750.11812697862689199No Hit
TAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC740.1165519522452001No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.11340189948181632No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC720.11340189948181632No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC710.11182687310012443No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC710.11182687310012443No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC710.11182687310012443No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC710.11182687310012443No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC700.11025184671843254No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC700.11025184671843254No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC690.10867682033674064No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC680.10710179395504874No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC680.10710179395504874No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC680.10710179395504874No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC680.10710179395504874No Hit
CAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC680.10710179395504874No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC670.10552676757335687No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC670.10552676757335687No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC670.10552676757335687No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC670.10552676757335687No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC660.10395174119166496No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.10395174119166496No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC660.10395174119166496No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC650.10237671480997307No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC650.10237671480997307No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC650.10237671480997307No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC640.10080168842828117No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC640.10080168842828117No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)