FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81489
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC830.10185423799531225No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC830.10185423799531225No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC830.10185423799531225No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCTA1100.0295.000032
ATCCCTA1100.0295.000032
TTGCCCT550.0295.000031
TGATCCT400.0295.01
GCCCCTA600.0295.02
ATGGGAT157.21574E-6295.0295
AATCCTA900.0295.02
GTCGCCT450.0295.01
AGATCCT206.149821E-8295.01
CGAACCT500.0295.01
ACGCCTA800.0295.02
GTACAGT157.21574E-6295.0295
CAACGGG108.45062E-4295.06
CAGCCTA1000.0295.02
CCTAGGG350.0295.05
CGGCCTA750.0295.02
CCCTAGG108.45062E-4295.04
TAGACCT400.0295.01
GACACCT303.6379788E-12295.01
ACACCCT255.275069E-10295.01
Produced by FastQC (version 0.11.5)