FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76257
Sequences flagged as poor quality0
Sequence length301
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA68779.018188494171026No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA49736.521368530102155No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA40565.318855973877808No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA28953.7963727920059793No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA25703.370182409483719No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA17922.3499482014765856No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA15782.0693182265234666No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA14631.918512398861744No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA14591.913266978769162No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA12571.648373264093788No Hit
GGACTACAAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT9961.3061096030528345No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA9711.2733257274741991No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA8731.1448129352059484No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA8361.096292799349568No Hit
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG8061.0569521486552054No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA7891.0346591132617333No Hit
GGACTACAAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT7090.9297507114101001No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA6380.8366445047667755No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG6270.8222195995121759No Hit
GGACTACTAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT6090.7986152090955585No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA5200.6819046120356164No Hit
GGACTACTAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4930.6464980264106902No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA4830.633384476179236No Hit
GGACTACCAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT4190.5494577546979293No Hit
GGACTACTAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT4060.532410139397039No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3780.4956921987489673No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA3600.47208780833234976No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3520.46159696814718654No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3440.45110612796202315No Hit
GGACTACCAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3180.4170108973602423No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA3180.4170108973602423No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2840.3724248265732982No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2690.35275450122611696No Hit
GGACTACACGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2450.32128198067062697No Hit
GGACTACAGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2280.2989889452771549No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2200.28849810509199153No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2150.2819413299762645No Hit
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2110.2766959098836828No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2100.27538455486053737No Hit
GGACTACTCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2070.27145048979110115No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2060.27013913476795576No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC2010.26358235965222865No Hit
GGACTACAGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2010.26358235965222865No Hit
GGACTACAGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1970.258336939559647No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1960.2570255845365016No Hit
GGACTACCAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1880.24653474435133824No Hit
GGACTACCAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1860.24391203430504743No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1800.23604390416617488No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1780.23342119411988407No Hit
GGACTACTGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1690.22161899891157533No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1680.2203076438884299No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1650.21637357881899363No Hit
GGACTACAAGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1640.21506222379584825No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1640.21506222379584825No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1600.20981680370326655No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1580.20719409365697575No Hit
GGACTACAGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1580.20719409365697575No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1520.19932596351810325No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1450.19014647835608534No Hit
GGACTACTGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1360.17834428314777662No Hit
GGACTACTCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1340.17572157310148578No Hit
GGACTACAAGGGTATCTAACTGAAGGGGCGATTTCCCTTCACATGTCCAC1320.17309886305519492No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1310.17178750803204954No Hit
GGACTACAAGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1280.16785344296261326No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1200.15736260277744993No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1190.15605124775430454No Hit
GGACTACAAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1190.15605124775430454No Hit
GGACTACTGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1170.15342853770801368No Hit
GGACTACAAGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1140.14949447263857746No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1140.14949447263857746No Hit
GGACTACTGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1090.14293769752285038No Hit
GGACTACTAGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1080.14162634249970496No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1080.14162634249970496No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1050.13769227743026868No Hit
GGACTACCGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1040.1363809224071233No Hit
GGACTACCGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1040.1363809224071233No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1040.1363809224071233No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1020.13375821236083243No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1020.13375821236083243No Hit
GGACTACAAGGGTATCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA1010.13244685733768705No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1000.1311355023145416No Hit
GGACTACAAGGGTATCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG1000.1311355023145416No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC920.12064466212937829No Hit
GGACTACAAGGGTTTCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA870.1140878870136512No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC860.11277653199050579No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC840.11015382194421496No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC840.11015382194421496No Hit
GGACTACAAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA830.10884246692106955No Hit
GGACTACAAGGGTTTCTAACTGAAGGGGCGATTTCCCTTCACATGTCCAC830.10884246692106955No Hit
GGACTACAAGGGTATCTAATCTTGGCTGCACACTAGCATCACTCTGGTTT830.10884246692106955No Hit
GGACTACAAGGGTATCTAACATACGTGTACCAGGTTGGCATGCCTCCAGC820.10753111189792412No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC810.1062197568747787No Hit
GGACTACAAGGGTATCTAATGGTCCCTGGAGTTTCTCGGCACAGAGCTCA780.10228569180534247No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGG2850.0295.06
ATTAGCC108.449562E-4295.0295
CGGGGTA1000.0295.08
CTACCGG2900.0295.04
CTACCCG500.0295.04
TACCGGG2900.0295.05
GGACTAC72100.0294.795441
TACACGG4000.0291.31255
CTCGGGT3200.0290.390627
GACTACA37150.0289.83852
AGGGTAT26150.0288.79549
GACTACC11300.0288.473482
TCGGGTT2200.0288.295478
TACAAGG25800.0288.139565
CAAGGGT25700.0288.112857
ACAAGGG25700.0288.112856
GGGGTAT8550.0288.099439
ACACGGG4050.0287.716036
CACGGGT4000.0287.6257
ACTACAG7800.0287.435883
Produced by FastQC (version 0.11.5)