FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61656
Sequences flagged as poor quality0
Sequence length301
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT870.14110548851693266No Hit
TTTTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT840.13623978201634876No Hit
TTATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT830.13461787984948748No Hit
TTAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT790.1281302711820423No Hit
TAATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT790.1281302711820423No Hit
TCCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT790.1281302711820423No Hit
CTCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT760.12326456468145842No Hit
ACATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT760.12326456468145842No Hit
TAAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT750.12164266251459713No Hit
ATAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT750.12164266251459713No Hit
TCAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT750.12164266251459713No Hit
AATTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT740.12002076034773582No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.12002076034773582No Hit
TTCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT730.11839885818087452No Hit
AACTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT730.11839885818087452No Hit
AAATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT720.11677695601401324No Hit
CATACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT720.11677695601401324No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.11515505384715194No Hit
TAGTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT710.11515505384715194No Hit
ATCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT710.11515505384715194No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.11353315168029066No Hit
TTCTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT700.11353315168029066No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.11353315168029066No Hit
CACTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT700.11353315168029066No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.11191124951342936No Hit
ATATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT690.11191124951342936No Hit
TGGACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT680.11028934734656805No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.11028934734656805No Hit
ATCTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT680.11028934734656805No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.10866744517970675No Hit
CTCTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT670.10866744517970675No Hit
TCCTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT670.10866744517970675No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.10866744517970675No Hit
TCTACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT670.10866744517970675No Hit
GTTACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT670.10866744517970675No Hit
TTTACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT660.10704554301284547No Hit
ACCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT660.10704554301284547No Hit
TTGTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT660.10704554301284547No Hit
ACAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT650.10542364084598417No Hit
ATTACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT650.10542364084598417No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.10542364084598417No Hit
GTATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT650.10542364084598417No Hit
CCAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT650.10542364084598417No Hit
GTAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT640.10380173867912289No Hit
CTTTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT640.10380173867912289No Hit
AAAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT640.10380173867912289No Hit
ACCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10380173867912289No Hit
CTGACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT640.10380173867912289No Hit
TTGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10380173867912289No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10380173867912289No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10380173867912289No Hit
TGCTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT630.10217983651226158No Hit
CTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10217983651226158No Hit
GACACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT630.10217983651226158No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10217983651226158No Hit
TACTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT630.10217983651226158No Hit
AACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.10055793434540028No Hit
ATGTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT620.10055793434540028No Hit
ATGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.10055793434540028No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.10055793434540028No Hit
TACACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT620.10055793434540028No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.10055793434540028No Hit
TTACCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT620.10055793434540028No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCTA1150.0295.000032
TCGCCTA500.0295.02
GTTGATT157.2072417E-6295.0295
TCGCCCT303.6379788E-12295.01
GTACCCT255.2568794E-10295.01
ACGCCTA600.0295.02
ACGCCCT206.137816E-8295.01
GCGACCT350.0295.01
ATGGCCT255.2568794E-10295.01
CACGCCT255.2568794E-10295.01
ACGGGGC157.2072417E-6295.08
GCACCTA1200.0295.02
CGCCTAC2350.0295.03
AGGCCTA900.0295.02
ACCTAGG108.4456423E-4295.04
TACGCCT157.2072417E-6295.01
GTTCGGG108.4456423E-4295.0295
AACGCCT157.2072417E-6295.01
CGTACCT206.137816E-8295.01
ACGTCCT500.0295.01
Produced by FastQC (version 0.11.5)