FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46230
Sequences flagged as poor quality0
Sequence length301
%GC53

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA33247.190136275146008No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA29306.337875838200303No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA26975.8338741077222585No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA25085.425048669695003No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA23985.18710793856803No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA22474.860480207657365No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA22154.791261085874973No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA21204.585766818083496No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA20184.36513086740212No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA20164.360804672290721No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA18804.0666234047155525No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA17133.7053861129136925No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA17033.683755137356695No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA16243.5128704304564136No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA15503.3528012113346315No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA13822.989400821977071No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA12962.8033744321868914No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA11952.5849015790612153No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT8750.0295.000039
TACTCGG4400.0295.000035
AGTTCGC1100.0295.00003295
ACACGGG6150.0295.06
CGTTATC108.438797E-4295.0295
TACAGGG4900.0295.05
GTACTAC108.438797E-4295.01
GAAAATA108.438797E-4295.0295
AATTAGC206.121263E-8295.0295
AGGGTAC108.438797E-4295.09
TACACGG6200.0295.05
ACGGGTA3400.0295.08
TACTGGG3500.0295.05
TTTAGCC108.438797E-4295.0295
CCGGGTT2500.0295.08
CCGGGTA2850.0295.08
TCCCGTC108.438797E-4295.0295
ACTGGGG3450.0295.06
CTACCGG3550.0295.04
CTACCCG5350.0295.04
Produced by FastQC (version 0.11.5)