FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72656
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCCT550.0295.000031
GGCGCCT350.0295.000031
CGTCCTA950.0295.02
CGCGCCT303.6379788E-12294.999971
CGCCTCG108.4487436E-4294.999973
TACGCCT303.6379788E-12294.999971
CTACGGC108.4487436E-4294.999976
CTACCGG206.145274E-8294.999976
CGTACCT108.4487436E-4294.999971
CCGACCT157.212535E-6294.999971
CGGTCCT157.212535E-6294.999971
GCGTCCT303.6379788E-12294.999971
TCGACCT157.212535E-6294.999971
CTTACGG108.4487436E-4294.999975
GTCCTAC3950.0291.265843
CCCTACG15650.0291.230044
ACCTACG18200.0290.94784
TACGGGA65300.0290.25657
CCTACGG68100.0290.234955
CTACGGG66250.0289.879246
Produced by FastQC (version 0.11.5)