FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84247
Sequences flagged as poor quality0
Sequence length301
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1130.13412940520137215No Hit
TCATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1070.12700748988094532No Hit
TTTACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1020.12107256044725628No Hit
TTCTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1020.12107256044725628No Hit
AAAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1020.12107256044725628No Hit
CTATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1000.11869858867378066No Hit
ACAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1000.11869858867378066No Hit
TCAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT980.11632461690030506No Hit
TTTTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT970.11513763101356725No Hit
TTAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT970.11513763101356725No Hit
TAAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT960.11395064512682944No Hit
ATGTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT950.11276365924009163No Hit
TCACCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT940.11157667335335382No Hit
TTCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT930.11038968746661602No Hit
CAAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT930.11038968746661602No Hit
TTTCCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT920.10920270157987821No Hit
GTAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT900.1068287298064026No Hit
TTACCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT900.1068287298064026No Hit
ACATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT900.1068287298064026No Hit
TCTTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT900.1068287298064026No Hit
GTATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT900.1068287298064026No Hit
TTGACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT890.1056417439196648No Hit
TACACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT880.10445475803292699No Hit
ATTACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT880.10445475803292699No Hit
TATACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT880.10445475803292699No Hit
TCTACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT880.10445475803292699No Hit
CTCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT870.10326777214618918No Hit
ATACCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT870.10326777214618918No Hit
AAACCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT870.10326777214618918No Hit
ACGTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT860.10208078625945137No Hit
ATCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT860.10208078625945137No Hit
ATTTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT860.10208078625945137No Hit
ATCTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT860.10208078625945137No Hit
CTAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT860.10208078625945137No Hit
CCAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT860.10208078625945137No Hit
TAATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT850.10089380037271356No Hit
ATATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT850.10089380037271356No Hit
TACTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT850.10089380037271356No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA750.0295.02
TGCCCCT255.275069E-10295.01
AGATCCT350.0295.01
ACGCCTA1250.0295.02
GGTCCTA1250.0295.02
CCTACGC108.451126E-4295.05
ACGGGGC206.1510946E-8295.08
GTAGCCT350.0295.01
TACGCCT500.0295.01
TACGAGG108.451126E-4295.07
GGGCGGC108.451126E-4295.09
CCGACCT206.1510946E-8295.01
CGTCCTA1050.0295.02
ATCGCCT108.451126E-4295.01
ACGACCT206.1510946E-8295.01
ACGGGAA157.216606E-6294.999978
TCTACGG157.216606E-6294.999974
CGATCCT600.0294.999971
CCCTACG19150.0291.919074
TCCTACG23500.0291.86174
Produced by FastQC (version 0.11.5)