FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60472
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCTA1100.0295.000032
CCTCCTA1100.0295.000032
GTTGATT157.206547E-6295.0295
GCCCCTA800.0295.02
TCGCCCT157.206547E-6295.01
GTCGCCT157.206547E-6295.01
CCCCTAC3900.0295.03
TGCCCCT350.0295.01
AGATCCT157.206547E-6295.01
CGAACCT157.206547E-6295.01
ACGCCTA700.0295.02
ACGCCCT303.6379788E-12295.01
GCGACCT108.4452366E-4295.01
GTTTCCT255.2568794E-10295.01
CTATCCT303.6379788E-12295.01
AGCCCTA650.0295.02
GAGTCCT350.0295.01
TAGACCT255.2568794E-10295.01
GACACCT303.6379788E-12295.01
TGGACCT255.2568794E-10295.01
Produced by FastQC (version 0.11.5)