FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76882
Sequences flagged as poor quality0
Sequence length301
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCC990.12876876251918526No Hit
TCATCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCC970.1261673733773835No Hit
CACACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCC950.1235659842355818No Hit
TTTTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCC880.11446112223927576No Hit
TTCTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCC870.1131604276683749No Hit
TTAACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCC870.1131604276683749No Hit
ACATCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCC870.1131604276683749No Hit
TAATCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCC860.11185973309747405No Hit
ATCACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCC840.10925834395567233No Hit
TTATCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCC840.10925834395567233No Hit
TCTTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCC830.10795764938477147No Hit
TCTACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCC830.10795764938477147No Hit
CAAACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCC820.10665695481387061No Hit
TCAACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCC820.10665695481387061No Hit
TTACCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCC810.10535626024296976No Hit
TACACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCC810.10535626024296976No Hit
TTGACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCC800.10405556567206888No Hit
GTCACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCC800.10405556567206888No Hit
CACTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCC800.10405556567206888No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC790.10275487110116802No Hit
AACACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCC780.10145417653026716No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC780.10145417653026716No Hit
TTTACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCC780.10145417653026716No Hit
ATAACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCC770.1001534819593663No Hit
TCCACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCC770.1001534819593663No Hit
ATCTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCC770.1001534819593663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCTA1100.0295.000032
CAACCTA1550.0295.000032
TCGTCCT550.0295.000031
TCGCCTA850.0295.02
CCTTCCT750.0295.01
TCGCCCT255.2568794E-10295.01
GCCCCCT206.1476385E-8295.01
ACAACCT450.0295.01
CGAACCT400.0295.01
TCACCCT400.0295.01
CAGCCTA450.0295.02
GTCCTAG108.449693E-4295.03
GAGTATC108.449693E-4295.0295
GGTCCTA950.0295.02
TTGTCCT206.1476385E-8295.01
GATCCCT255.2568794E-10295.01
GTCTCGT206.1476385E-8295.0295
GTCTCCT400.0295.01
CTCCCCT108.449693E-4295.01
GGTCCCT255.2568794E-10295.01
Produced by FastQC (version 0.11.5)