FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005491371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81859
Sequences flagged as poor quality0
Sequence length301
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1030.1258261156378651No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1000.12216127731831564No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC970.11849643899876616No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC920.1123883751328504No Hit
CCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC920.1123883751328504No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC900.10994514958648408No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC900.10994514958648408No Hit
AACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC890.10872353681330094No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC890.10872353681330094No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC870.1062803112669346No Hit
AAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC870.1062803112669346No Hit
GTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC860.10505869849375143No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC860.10505869849375143No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC840.10261547294738514No Hit
ACTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC830.10139386017420197No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC820.10017224740101884No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC820.10017224740101884No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)