FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104104
Sequences flagged as poor quality0
Sequence length301
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1360.13063859217705373No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1290.12391454699147007No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1280.12295396910781525No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1270.12199339122416046No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1260.12103281334050564No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1260.12103281334050564No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1250.12007223545685085No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1240.11911165757319603No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1240.11911165757319603No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1240.11911165757319603No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1230.11815107968954122No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1230.11815107968954122No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1220.11719050180588643No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1200.11526934603857682No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1190.114308768154922No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1170.1123876123876124No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1160.11142703450395758No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1150.11046645662030279No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1130.10854530085299316No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1130.10854530085299316No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1130.10854530085299316No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1120.10758472296933834No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1120.10758472296933834No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1120.10758472296933834No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1110.10662414508568355No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1110.10662414508568355No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1110.10662414508568355No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1100.10566356720202875No Hit
TTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1090.10470298931837393No Hit
CGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1080.10374241143471913No Hit
CCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1080.10374241143471913No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1080.10374241143471913No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1080.10374241143471913No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1080.10374241143471913No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1080.10374241143471913No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1080.10374241143471913No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1080.10374241143471913No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1080.10374241143471913No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1070.10278183355106431No Hit
GGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1070.10278183355106431No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1070.10278183355106431No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1060.10182125566740952No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1050.1008606777837547No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1050.1008606777837547No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC1050.1008606777837547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCCTA1700.0295.000032
TCGCCTA1350.0295.02
GCCCCTA950.0295.02
ATCCTCG108.4539934E-4295.03
AATCCTA1750.0295.02
ATCTCCT500.0295.01
CCCCTAG108.4539934E-4295.03
CGAACCT255.275069E-10295.01
TCACCTA2550.0295.02
CCCTCCT350.0295.01
TCACCCT255.275069E-10295.01
CAACGGG206.158007E-8295.06
CTTCCCT255.275069E-10295.01
CCTAGGG750.0295.05
GAGTCCT350.0295.01
CCCTAGG206.158007E-8295.04
ACCGCCT400.0295.01
GATCCTA1500.0295.02
ATTACCT650.0295.01
TTGTCCT550.0295.01
Produced by FastQC (version 0.11.5)