FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65551
Sequences flagged as poor quality0
Sequence length301
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCTA1100.0295.000032
GATCCTA1050.0295.02
TACGGGC108.44686E-4295.07
GTTACCT400.0295.01
CCCCCTA650.0295.02
GACGCCT255.2568794E-10295.01
CGTCCTA1000.0295.02
TTCGCCT108.44686E-4295.01
CGCGCCT303.6379788E-12294.999971
TCCTACG16300.0293.19024
CCTACGG60650.0291.352025
CTACGGG59550.0290.541566
GCCTACG12600.0290.317444
ACCTACG18350.0290.177124
CCCTACG13550.0289.55724
TACGGGA58550.0289.20587
ACGGGAG58850.0288.483438
CGCCTAC3650.0286.91783
CGGGAGG57750.0285.294379
GTCCTAC4250.0284.588233
Produced by FastQC (version 0.11.5)