FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005491392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65551
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7341.1197388293084773No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6991.0663452884013973No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6761.0312581043767448No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6701.022104925935531No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5430.828362648929841No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5180.7902244054247838No Hit
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4740.7231010968558832No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4710.7185245076352763No Hit
GGACTACTCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4510.6880139128312307No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4440.6773352046498147No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4330.6605543775075895No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4280.6529267288065781No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4090.6239416637427346No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3900.5949565986788913No Hit
GGACTACCCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3640.5552928254336318No Hit
GGACTACTGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3520.5369864685512044No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3420.5217311711491815No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3020.4607099815410901No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2930.44698021387926956No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA2770.42257173803603304No Hit
GGACTACCGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2730.41646961907522384No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2660.4057909108938078No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2460.37528031608976214No Hit
GGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA2430.3707037268691553No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2430.3707037268691553No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2400.36612713764854843No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2370.3615505484279416No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA2360.3600250186877393No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA2340.35697395920733477No Hit
GGACTACTCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2340.35697395920733477No Hit
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2290.3493463105063233No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2270.34629525102591874No Hit
GGACTACACGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2190.3340910131043005No Hit
GGACTACTAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2150.32798889414349136No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2110.3218867751826822No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2070.3157846562218731No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1960.2990038290796479No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1960.2990038290796479No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1910.29137618037863644No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA1910.29137618037863644No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1850.2822230019374228No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1850.2822230019374228No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1800.2745953532364114No Hit
GGACTACTGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1780.27154429375600675No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1710.2608655855745908No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1710.2608655855745908No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA1710.2608655855745908No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1560.2379826394715565No Hit
GGACTACTCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1560.2379826394715565No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1530.23340605025094965No Hit
GGACTACTCGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1510.23035499077054505No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1490.2273039312901405No Hit
GGACTACTAGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1490.2273039312901405No Hit
GGACTACACGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1480.22577840154993822No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1480.22577840154993822No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1480.22577840154993822No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1450.22120181232933137No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1440.21967628258912908No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1440.21967628258912908No Hit
GGACTACCGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1430.2181507528489268No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA1410.21509969336852222No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1360.2074720446675108No Hit
GGACTACAAGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1350.2059465149273085No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1340.20442098518710625No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1340.20442098518710625No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1330.2028954554469039No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA1300.1983188662262971No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1300.1983188662262971No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1290.19679333648609482No Hit
GGACTACTGGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1280.19526780674589253No Hit
GGACTACTAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1270.19374227700569022No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1260.19221674726548793No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1240.18916568778508336No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA1220.1861146283046788No Hit
GGACTACACGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1220.1861146283046788No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1210.18458909856447653No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1210.18458909856447653No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1170.17848697960366738No Hit
GGACTACCCGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1170.17848697960366738No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1160.17696144986346507No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA1160.17696144986346507No Hit
GGACTACCGGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1150.1754359201232628No Hit
GGACTACCCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1130.17238486064285824No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1090.1662827416820491No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1060.16170615246144224No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA1020.1556040335006331No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1020.1556040335006331No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1020.1556040335006331No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1020.1556040335006331No Hit
GGACTACAAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1020.1556040335006331No Hit
GGACTACTGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1010.1540785037604308No Hit
GGACTACAGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1010.1540785037604308No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1010.1540785037604308No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA970.14797638479962166No Hit
GGACTACCAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG940.14339979557901483No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA920.14034873609861026No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA910.13882320635840795No Hit
GGACTACTCGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC900.1372976766182057No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA890.13577214687800337No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA890.13577214687800337No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA890.13577214687800337No Hit
GGACTACTAGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC880.1342466171378011No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA870.13272108739759883No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA870.13272108739759883No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA850.12967002791719426No Hit
GGACTACAAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT840.12814449817699194No Hit
GGACTACAAGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC830.1266189684367897No Hit
GGACTACCGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT830.1266189684367897No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA810.12356790895638511No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA800.12204237921618281No Hit
GGACTACTCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT800.12204237921618281No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA780.11899131973577826No Hit
GGACTACACGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC750.11441473051517138No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA740.11288920077496911No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC740.11288920077496911No Hit
GGACTACCCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG740.11288920077496911No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA730.11136367103476681No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA730.11136367103476681No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA730.11136367103476681No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA720.10983814129456454No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC710.10831261155436224No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA680.1037360223337554No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)