FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46783
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC530.11328901524057884No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC510.10901395806168906No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC500.10687642947224418No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC500.10687642947224418No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC500.10687642947224418No Hit
AACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC490.10473890088279932No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC490.10473890088279932No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC480.10260137229335443No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC480.10260137229335443No Hit
TAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC470.10046384370390954No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC470.10046384370390954No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT206.121991E-8295.01
TCGCCTA700.0295.02
AATCCTA700.0295.02
CAAACCT303.6379788E-12295.01
AGATCCT303.6379788E-12295.01
ACGCCTA750.0295.02
GCGACCT157.1961094E-6295.01
TCCGCCT157.1961094E-6295.01
CTATCCT108.4391184E-4295.01
GATCCTA1000.0295.02
CTCCCTA750.0295.02
TTGTCCT157.1961094E-6295.01
GTCTCCT303.6379788E-12295.01
ATAGCCT255.2386895E-10295.01
CGACCTA500.0295.02
CACGCCT303.6379788E-12295.01
CGCGCCT206.121991E-8295.01
TGAACCT255.2386895E-10295.01
GCGCCTA500.0295.02
CCTACGC108.4391184E-4295.05
Produced by FastQC (version 0.11.5)