FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70531
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC750.10633622095248899No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.10350058839375594No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC720.10208277211438942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCCT400.0295.01
ATCCCCT500.0295.01
AAACCTA1850.0295.02
GTTGATT206.1440005E-8295.0295
GTTTGTC108.448213E-4295.0295
TTGTCCT303.6379788E-12295.01
TGGTCCT303.6379788E-12295.01
ACGCCTA550.0295.02
TGCACCT157.211629E-6295.01
GGGTCCT350.0295.01
GCCTCCT108.448213E-4295.01
GGCACCT255.2568794E-10295.01
CCTACCA108.448213E-4295.01
TATACCT303.6379788E-12295.01
TCGACCT303.6379788E-12295.01
CGCCCTA700.0295.02
GAAACCT206.1440005E-8295.01
TTCGCCT157.211629E-6295.01
GATACCT255.2568794E-10295.01
TCCTAGG206.1440005E-8295.04
Produced by FastQC (version 0.11.5)