FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79842
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCCT350.0295.000031
GATTCCT350.0295.000031
CGAACCT303.6379788E-12295.01
CAACGGG108.450299E-4295.06
CCTACGA157.2151925E-6295.05
CGTTCCT157.2151925E-6295.01
TCCTAGG157.2151925E-6295.04
GCACCCT400.0295.01
GTAGCCT206.149094E-8295.01
CGCTCCT206.149094E-8295.01
CTACGAG157.2151925E-6295.06
GACGCCT303.6379788E-12295.01
CGGTCCT303.6379788E-12295.01
CGCACCT400.0295.01
ATCGCCT157.2151925E-6295.01
GCTCCTA750.0295.02
CCCGCCT108.450299E-4295.01
GTGCCTA400.0295.02
CCCTACG16200.0292.268524
GCCTACG12700.0291.515754
Produced by FastQC (version 0.11.5)