FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99859
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1150.11516237895432559No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1090.10915390700888253No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1010.10114261108162509No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1000.1001411990907179No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1000.1001411990907179No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1000.1001411990907179No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAACCT550.0295.000031
TTCGCCT550.0295.000031
ATCGCCT550.0295.000031
TGATCCT500.0295.01
TCGCCTA1600.0295.02
GCCCCTA1400.0295.02
ATCCTCG108.453477E-4295.03
GTCGCCT255.275069E-10295.01
AGATCCT450.0295.01
CGAACCT500.0295.01
GTACAGT108.453477E-4295.0295
CAACGGG255.275069E-10295.06
GCAACCT500.0295.01
GATCCTA1800.0295.02
CGCGCCT255.275069E-10295.01
CCTACGA255.275069E-10295.05
GCGCCCT350.0295.01
CGCGAAA206.156915E-8295.0295
GACCCCT400.0295.01
AGAACCT400.0295.01
Produced by FastQC (version 0.11.5)