FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68653
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1010.147116659140897No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1000.14566005855534353No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC910.1325506532853626No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC910.1325506532853626No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC880.12818085152870232No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC860.12526765035759543No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC850.12381104977204202No Hit
CTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC850.12381104977204202No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC850.12381104977204202No Hit
CATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC840.12235444918648856No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC840.12235444918648856No Hit
CATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC830.12089784860093514No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC830.12089784860093514No Hit
CACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC830.12089784860093514No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.11798464742982827No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.11652804684427483No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC790.1150714462587214No Hit
ATGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC790.1150714462587214No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.11361484567316796No Hit
GGCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.11361484567316796No Hit
ACTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.11361484567316796No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.11361484567316796No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.11361484567316796No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.11361484567316796No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.11215824508761453No Hit
GTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.11215824508761453No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.11215824508761453No Hit
TATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.11215824508761453No Hit
AAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.11070164450206108No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.11070164450206108No Hit
AACCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC750.10924504391650765No Hit
GCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC750.10924504391650765No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC750.10924504391650765No Hit
AACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.10778844333095422No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.10778844333095422No Hit
CTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.10778844333095422No Hit
TCGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.10487524215984734No Hit
TAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.10487524215984734No Hit
GAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.10487524215984734No Hit
CCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.10341864157429391No Hit
ATTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.10341864157429391No Hit
ACACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.10341864157429391No Hit
GTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.10341864157429391No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.10196204098874048No Hit
AAGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.10196204098874048No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.10050544040318704No Hit
TGAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.10050544040318704No Hit
AGGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.10050544040318704No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.10050544040318704No Hit
TATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.10050544040318704No Hit
CCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.10050544040318704No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCTA700.0295.000032
CGTTCCT350.0295.000031
TACGGGG900.0295.07
CGTACCT255.2568794E-10295.01
GAACCTA1100.0295.02
CGTCCTA1150.0295.02
TAAGCCT255.2568794E-10295.01
CCGCCTA1100.0295.02
ATACCTA1350.0295.02
ACGACCT255.2568794E-10295.01
CCCGCCT255.2568794E-10295.01
CGGACCT550.0295.01
GCCCCCT157.210807E-6294.999971
GTCGCCT206.142909E-8294.999971
ACGCCCT157.210807E-6294.999971
GCGCCTA800.0294.999972
GCGCCCT157.210807E-6294.999971
TACGGCG108.447731E-4294.999977
CGCCTAG108.447731E-4294.999973
ACCTAGG157.210807E-6294.999974
Produced by FastQC (version 0.11.5)