FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82359
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT500.0295.01
GCCCCTA1250.0295.02
GTCGCCT303.6379788E-12295.01
CCCCTAG157.216029E-6295.03
CGAACCT350.0295.01
GCGACCT108.4507896E-4295.01
CTTCCCT450.0295.01
GAGTCCT157.216029E-6295.01
TAGACCT303.6379788E-12295.01
GCGCGGA108.4507896E-4295.0295
ATGGACA255.275069E-10295.0295
GATCCTA1650.0295.02
GGTCCTA1350.0295.02
CGCGCCT206.150367E-8295.01
GGATCCT206.150367E-8295.01
CGCGAAA157.216029E-6295.0295
ACGGGGC303.6379788E-12295.08
GACCCCT700.0295.01
GCACCTA1800.0295.02
ATTCCTA1250.0295.02
Produced by FastQC (version 0.11.5)