FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87963
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGG206.152732E-8295.000035
GTCACCT206.152732E-8295.000031
CACACCT400.0295.000031
GTGCACA206.152732E-8295.00003295
CTCCCTA850.0295.000032
GAAACCT108.451764E-4295.000031
GAGTGGG400.0295.00003295
TGCGCCT108.451764E-4295.000031
GATACCT400.0295.000031
GTCGCCT400.0295.000031
CCTGCCT108.451764E-4295.000031
TCGGCCT400.0295.000031
CATCCCT206.152732E-8295.000031
CGCTCCA108.451764E-4295.000031
CTCCACG108.451764E-4295.000033
GTACAGG108.451764E-4295.00003295
GTTTCCT206.152732E-8295.000031
ACTCCCT108.451764E-4295.000031
CCTTGAT206.152732E-8295.00003295
TCTACGG206.152732E-8295.000034
Produced by FastQC (version 0.11.5)